外源性一氧化氮对骨肉瘤细胞株表面超微结构的影响  

The Influence of Ectogenous Nitric Oxide on the Ultrastructure of Osteosarcoma Cell Line

作　　者：张新宇[1] 靳安民[1] 安连兵[2] 殷铭东[3] 卢萍[4] 

机构地区：[1]第一军医大学珠江医院脊柱骨科,广州510282 [2]第一军医大学基础部电镜室,广州510515 [3]解放军第205医院骨科,锦州121001 [4]解放军第205医院信息科,锦州121001

出　　处：《中国矫形外科杂志》2002年第5期473-475,共3页Orthopedic Journal of China

摘　　要：目的 :研究外源性一氧化氮 (NO)作用于体外培养的骨肉瘤细胞株 (HOS)后细胞表面形态的变化 ,推测其对HOS侵袭能力的影响。方法 :在HOS培养液中加入不同浓度的硝普钠 (SNP)以产生外源性的NO ,通过扫描电镜 ,观察骨肉瘤细胞株表面形态的超微结构变化。结果 :不同浓度的SNP在体外均可改变细胞表面形态 ,浓度越高 ,细胞表面的绒毛或突起越少 ,伸展范围也越小 ,而对照组细胞表面的绒毛突起丰富且向周围伸展。结论 :NO可以减少骨肉瘤细胞株表面的绒毛突起 。Objective: To study the influences of exogenous nitric oxide on the formation of villus or processus of human osteosarcoma cell line (HOS).Methods:Different concentrations of sodium nitroprusside(SNP)were added into the culture solution of HOS in vitro.Through scanning electron microscope,the villus or processus in the surface of HOS were observed.Results:All the groups of SNP could depress the formation of villus or processus of HOS.The difference was also obvious.The more concentration of SNP was the less formation of villus or processus.Meanwhile the formation of villus or processus and extension in the control group (PBS)were very obvious.Conclusions:Exogenous nitric oxide could depress the formation of villus or processus of HOS in vitro.Thos phenomenon might direct the losses of invasive and metastatic abilities of HOS in vitro.

关 键 词：一氧化氮 骨肉瘤 硝普钠 超微结构 

分 类 号：R738.1[医药卫生—肿瘤]