hTERT激活人成牙本质细胞端粒酶的实验研究  

Experimental study of hTERT activating telomerase in human odontoblast

作　　者：王捍国[1] 肖明振[1] 赵守亮[1] 郝建军[1] 高杰[1] 

机构地区：[1]第四军医大学口腔医学院,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2002年第3期118-120,共3页Chinese Journal of Conservative Dentistry

基　　金：全军"十五"计划基金重点课题 ( 0 12 0 89);国家自然科学基金资助项目 ( 39970 792 );教育部回国人员启动基金资助项目 ( 2 0 0 0HG0 0 3)

摘　　要：目的 :明确外源性人端粒酶逆转录酶 (humantelomerasereversetranscriptase,hTERT)能否调控人成牙本质细胞的端粒酶活性。方法 :培养人成牙本质细胞样细胞 ,采用脂质体转染法 ,将编码hTERT基因的真核表达质粒pCIneo -hTERT导入目的细胞。培养液加G418筛选 ,稳定后检测hTERT在mRNA和蛋白水平的表达以及端粒酶活性。结果 :转染后细胞表达hTERTmRNA及其蛋白 ,同时端粒酶活性转为阳性。结论 :在人成牙本质细胞样细胞内 ,外源性hTERT可以激活端粒酶 ,为建立永生化细胞系奠定了基础。AIM: To explicate whether the telomerase activity is regulated by human telomerase reverse transcriptase(hTERT)in human odontoblast. METHODS: Human odontoblast-like cells were cultured and transfected with eukaryotic expressing plasmid pCIneo-hTERT encoding hTERT . After selection with G418 to stablize the transfection, expression of hTERT mRNA and protein , and telomerase activity in untransfected and transfected cells were cultured. RESULTS: After stable transfection, hTERT was expressed at mRNA and protein level, and meanwhile telomerase activity was positive in these transfected cells. CONCLUSION: In human odontoblast-like cells, telomerase is activated by exogenous hTERT. This is the first step to establish immortalized human odontoblast cell line.

关 键 词：实验研究 HTERT 端粒酶 成牙本质细胞 

分 类 号：R780.2[医药卫生—口腔医学]