hTERT基因反义核酸对Hela宫颈癌细胞端粒酶活性的影响  

ATTENUATION OF TELOMERASE ACTIVITY BY USING AN ANTISENSE OLIGONUCLEOTIDE AGAINST hTERT mRNA IN CERVICAL CANCER CELLS.

作　　者：韩风华[1] 辛晓燕[1] 杜辉[1] 王健[1] 

机构地区：[1]第四军医大学西京医院,西安710032

出　　处：《西南国防医药》2002年第1期7-10,共4页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的 :研究人类端粒酶催化亚单位 (hTERT)基因的反义寡核苷酸对宫颈癌细胞的影响。方法 :采用TRAP -ELISA检测Hela细胞在反义核酸处理前后端粒酶活性的变化。结果 :hTERT基因反义核酸能有效抑制Hela细胞端粒酶活性。结论 :hTERT基因反义核酸能显著抑制肿瘤细胞的端粒酶活性 ,为肿瘤治疗提供了一个有效的新途径。Objective: To study the effects of antisense oligonucleotide against human telomerase catalytic subunit mRNA(hTERT mRNA)on human telomerase activity in cervical cancer cells in vitro. Method :Telomerase activity of cultured cervical cancer cells(Hela cells) was measured using the TRAP-ELISA assay before and after antisense treatment. Result: The antisense treatment could effectively inhibite the telomerase activity of Hela cells. Conclusion: Antisense oligonucleotide direct against hTERT mRNA could effectively inhibite the telomerase activity of tumor cells, which show an effective and novel approach for treatment of cancers.

关 键 词：HTERT基因 反义核酸 HELA 宫颈癌 细胞 端粒酶 活性 端粒酶催化亚单位 反义治疗 

分 类 号：R737.33[医药卫生—肿瘤]