维甲酸调变早幼粒白血病细胞株NB4中G-CSF及其受体表达  

Regulation of G - CSF and Its Receptor Expression in NB4 Cells by Retinoic Acid

作　　者：李俊娥[1] 孙关林[1] 邬维礼[1] 吴 文[1] 

机构地区：[1]上海第二医科大学瑞金医院上海血液研究所,上海200025

出　　处：《上海第二医科大学学报》2002年第3期193-196,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家"九五"医学科技攻关课题基金(969060117);国家自然科学基金(39470318);上海血液研究所胡应洲基金部分资助

摘　　要：目的进一步研究G-CSF在维甲酸致高白细胞症中的作用。方法用RT-PCR、Northernblotting方法检测NB4细胞经全反式维甲酸（ATRA）作用后 G—CSF mRNA表达的变化,同时用ELISA方法检测细胞培养上清液中G—CSF蛋白水平的变化;采用流式细胞技术检测NB4细胞经ATRA作用后G-CSF受体蛋白表达的变化。结果 ATRA可从基因水平上调NB4细胞中G-CSF表达,并可增加NB4细胞表面G—CSF受体的表达,G-CSF受体表达增加出现的时间虽然稍晚于G-CSF表达增加出现的时间,但仍然在较早期阶段,此时,细胞形态学和NBT实验均证实NB4细胞处于部分分化阶段。结论在ATRA治疗APL过程中,G—CSF及其受体表达增加可能协同参与了高白细胞症的发生。To further study the effect of G- CSF on the development of hyper-leukocytosis during inducting differentiation therapy with all - trans retinoic acid for acute promyelocytic leukemia. Methods The dynamic changes of G - CSF mRNA in NB4 cells co - cultured with ATRA was detected by means of RT - PCR and Northernblotting. Meanwhile,The dynamic changes of G- CSF protein in the supernatant of NB4 cells co - cultured with ATRA was detected using ELISA. The dynamic changes of G - CSF receptor expression on NB4 cells surface was detected by fluorescence - activated cell sorting analysis. Results ATRA transcriptionally up- regulated the expression of G- CSF in NB4 cells, the highest concentration of G - CSF in the supernatant was 120 percent of the control. ATRA could also up - regulate the expression of G - CSF receptor on the NB4 cell surface, the highest CD114 positive proportion of NB4 cell was eleven fold higher than the control. The expression of G -CSF receptor increased behind that of G- CSF 48h and 72h respectively, However, the increase in time occured in the early stage of NB4 cell differentiation as verified by the morphology and NET reduction test. These results indicated that there might be synergism between G- CSF and its receptor in promoting NB4 cells proliferation following their differentiation. Conclusion During inducting differentiation therapy with ATRA for APL,G- CSF and its receptor may synergetically contribute to the development of hyperleukocytosis.

关 键 词：早幼粒白血病 细胞株 NB4 G-CSF 全反式维甲酸 细胞因子 

分 类 号：R733.7[医药卫生—肿瘤] R977.21[医药卫生—临床医学]