胃癌中p21^(WAF1)与p53基因的相关性研究  

Relationship between expression of p21^(WAF1) and p53 gene in gastric carcinoma

作　　者：苏琦[1] 谢海龙[2] 凌晖[1] 罗招阳[1] 周建国[1] 李一琴[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001 [2]中南大学肿瘤研究所,湖南长沙410078

出　　处：《实用肿瘤杂志》2002年第3期158-161,共4页Journal of Practical Oncology

基　　金：湖南省卫生厅重点项目科研基金资助 (N0 .930 1)

摘　　要：目的　探讨胃癌中 p2 1WAF 1与 p5 3基因的关系。方法　采用 S- P法检测 p2 1WAF 1和 p5 3在胃癌中的表达。结果　 p2 1与 p5 3在正常胃黏膜、不典型增生、胃癌中的阳性表达率分别为 10 0 .0 %与 0 %、92 .5 %与 15 .0 %、39.8%与 5 6 .5 % ;40例不典型增生 ,p5 3阳性而 p2 1阴性者为 5 .0 % ,p5 3阴性而 p2 1阳性为 82 .5 % (P<0 .0 5 ) ;p2 1、p5 3阳性高分化腺癌各为 6 3.3%与 36 .7% ,与低分化腺癌 32 .7%与 78.2 %比较 ,差异有显著性 (P<0 .0 5 ) ;48.1%的胃癌 p2 1、p5 3蛋白表达相反 (P>0 .0 5 ) ;p2 1表达在侵犯浅肌层为 6 0 .0 %显著高于深肌层的 35 .2 % (P<0 .0 5 ) ;p2 1、p5 3在原发与转移癌中一致表达为 35 .3%与 70 .6 % ,皆与未转移癌 6 2 .5 %与 42 .5 %有显著性差异 (P<0 .0 5 ) ;p2 1在 TNM 期 6 0 .0 %、 期 5 6 .2 %显著高于 期 2 7.8%、 期 2 2 .2 % (P<0 .0 5 )。结论　正常胃黏膜和不典型增生 p2 1蛋白表达大部分依赖 p5 3蛋白 ,而胃癌在相当程度上不依赖。p2 1失表达和 p5 3异常表达与胃癌发生、分化程度、转移有关 ,而 p2 1失表达还与侵袭。Objective To investigate the relationship of expression of p21 WAF1 and p53 gene in gastric carcinoma. Methods The expression of p21 WAF1 and p53 protein was examined by streptavidin-peroxidase conjugated method (S-P) in gastric carcinoma. Results The positive expression rate of p21 and p53 were found in 100% and 0% of normal mucosa, in 92.5% and 15.0% of dysplasia and in 39.8% and 56.5% of gastric carcinoma,respectively. There were 5.0% cases who had a p53 positivity and p21 negativity, whereas 82.5% cases had a p53 negativity and p21 positivity in total 40 cases of dysplasia (P<0.05). The expression of p53 increased from well-differentiated (36.7%) to poorly-differentiated (78.2%) (P<0.05), while that of p21 decreased from (63.3%) to (32.7%) in 108 cases of gastric carcinoma(P<0.05), and they expressed reversely in 48.1% of gastric carcinoma. The expression of p21 in superficial muscle invasion (60.0%) was lower than that in deep muscle invasion (35.2%)(P<0.05). The expression of p21 and p53 in paired primary and lymph node metastasis carcinoma (35.3% and 70.6%)coincidently, different from non-metastasis carcinoma (62.5% and 42.5%) significantly (P< 0.05). The expression of p21 in TNM staging Ⅰ (60.0%) and Ⅱ (56.2%) was higher than that in Ⅲ (27.9%) and Ⅳ ( 22.2%) (P<0.05), whereas the expression of p53 did not correlate to the TNM staging. Conclusion The expression of p21 WAF1 protein depends on p53 protein largely in normal gastric mucosa and dysplasia, but not considerably in gastric carcinoma. The loss of p21 expression and abnormal expression of p53 is related to carcinogenesis, differentiation and metastasis, and the loss of p21 expression is related to invasion and clinical staging in gastric carcinoma intimately.

关 键 词：胃癌 P21^WAF1 P53基因 S-P法 肿瘤转移 

分 类 号：R735.2[医药卫生—肿瘤]