褪黑素主动免疫对雄兔睾丸发育的影响  

Effect of active melatonin immunization on development of rabbit testicle

作　　者：汪兴生[1] 张勇[2] 尚平[3] 夏银[4] 姜勋平[4] 韦来红[4] 程瑞禾[4] 沈子龙[1] 

机构地区：[1]中国药科大学生物技术中心,南京210009 [2]甘肃农业大学动物医学院 [3]解放军南京军区总医院 [4]南京农业大学动物科技学院

出　　处：《第二军医大学学报》2002年第5期559-561,共3页Academic Journal of Second Military Medical University

基　　金：江苏省自然科学基金资助项目 (BK970 95 )

摘　　要：目的 :研究褪黑素 (melatonin,Mel)与雄兔睾丸发育之间的关系。方法 :30只未达初情期的雄性新西兰大耳兔 ,随机分成 2组 ,每组 15只。实验组采用皮内多点多次注射 Mel与牛血清白蛋白偶联制备的完全抗原进行主动免疫 ,对照组相应注射等量的蒸馏水。实验期间 ,用 RIA法检测兔血清睾酮及促黄体生成激素含量 ,并在实验结束时测定兔睾丸、附睾质量及精子数。 结果 :进入发情期后 ,实验组兔血清睾酮及促黄体生成激素含量均明显低于对照组 (P<0 .0 1) ,睾丸、附睾质量及精子数亦显著低于对照组 (P<0 .0 1)。 结论 :通过Objective: To investigate the relationship between the melatonin and the development of testicle in rabbits. Methods: Thirty male rabbits (unreach puberty) were randomly divided into 2 groups:15 were actively immunized with melatonin and complete antigen, which was successfully synthesized through Mannich reaction by combining melatonin with BSA, the other 15 were taken as controls.The specific antibodies of rabbits were detected by ELISA and RIA.The serum testosterone(T) and luteinizing hormone(LH) were detected by RIA, the mass of testicle or epididymis and the number of sperm were measured. Results: The serum T and LH concentration in experiment group were significantly lower than that in the control group.The mass of testicle or epididymis and the number of sperm in experiment group were also significantly lower than that in the control group. Conclusion: The results suggest that the immunization of melatonin can block the development of testicle in male rabbits.

关 键 词：褪黑素 主动免疫 睾丸发育 动物实验 

分 类 号：R339.21[医药卫生—人体生理学] R977.1[医药卫生—基础医学]