环境内分泌干扰物诱发小鼠睾丸细胞DNA损伤的研究  

Study on DNA damage of spermary cell in mice induced by environmental endocrine disrupting chemicals

作　　者：孔志明[1] 张国栋[1] 孙立伟[1] 刘征涛[2] 

机构地区：[1]南京大学环境学院污染控制与资源化研究国家重点实验室,南京210093 [2]中国环境科学研究院,北京100012

出　　处：《环境污染与防治》2002年第2期76-78,共3页Environmental Pollution & Control

基　　金：国家自然科学基金资助项目 (No.2 99770 2 0 )

摘　　要：应用单细胞凝胶电泳技术 ,研究了烷基酚类化合物对离体小鼠睾丸细胞 DNA的损伤作用。实验结果表明 ,9种烷基酚类化合物对小鼠睾丸细胞均能引起不同程度的 DNA损伤 ,并呈现剂量效应关系。其高剂量组与对照组相比 ,均有极显著性差异 (P<0 .0 1)。实验结果亦表明 ,其The single cell gel electrophoresis technique (SCGE) was used to detecte the DNA damage of spermary cell in mice, which were induced by 9 tested environmental endocrine disrupting chemicals. The results show that all the nine tested substances can induce different levels DNA damages, and the dose response relation were obvious. The high dose group and the control group were significantly different (P<0.01). It is also showed that the degree of DNA damages were partly related to the structure of alkyl hydroxybenzene compound.

关 键 词：环境内分泌干扰物 小鼠睾丸细胞 DNA损伤 单细胞凝胶电泳 

分 类 号：R394.6[医药卫生—医学遗传学]