东亚钳蝎毒生物提取物对人肝癌细胞体外增殖及凋亡的影响  

EFFECT OF POLYPEPTIDE PURIFIED FORM BUTHUS MARTENSI KIRSCH(PBMK) ON PROLIFERATION AND APOPTOSIS IN HUMAN HEPATOMA CELL LINE

作　　者：杨春旭[1] 戴盛明[1] 朱灵[1] 

机构地区：[1]广西医科大学第四附属医院肿瘤科,生物技术研究所,柳州545005

出　　处：《广西医科大学学报》2002年第2期170-173,共4页Journal of Guangxi Medical University

摘　　要：目的 :探讨东亚钳蝎毒生物提取物 (PBMK)对人肝癌细胞的体外增殖及凋亡影响。方法 :用 MTT法测定 PBMK对人肝癌细胞的体外增殖 ,用 Tunel法测定其对人肝癌细胞的凋亡影响。结果 :PBMK对人肝癌细胞体外生长有明显抑制作用 ,可高度诱导肝癌细胞的凋亡。经 PBMK作用的癌细胞在相差显微镜下表现为细胞稀疏、收缩 ,胞质拉长。倒置显微镜下可见明显的细胞内空泡和细胞出泡现象。结论 :PBMK可作为一种有效的癌细胞毒药物 。Objectives: To investigate the effect of PBMK on proliferation and apoptosis in human hepatoma cell line in vitro Methods: MTT and Tunel detected the proliferation and apoptosis of human hepatoma cell line treated with PBMK respectively The morphology was observed by microscope Results: PBMK can significantly inhibit the growth of human hepatoma cell line and markedly induce the apoptosis of the human hepatoma line The hepatoma cells became small and vacuole Conclusion: It is suggested that PBMK have a promising prospect as an anti cancer drug

关 键 词：东亚钳蝎毒 生物提取物 蝎毒 肝癌细胞 细胞凋亡 细胞毒药物 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R965[医药卫生—临床医学]