人肝癌细胞膜胰岛素受体的研究及临床意义  

A study on insulin receptor on human hepatocellular cancer cell membrane

作　　者：程中[1] 欧小红[2] 匡安仁[2] 

机构地区：[1]四川大学华西医院普外科,成都610041 [2]四川大学华西医院核医学科,成都610041

出　　处：《中华普通外科杂志》2002年第6期340-342,共3页Chinese Journal of General Surgery

基　　金：国家自然科学基金资助项目 (3 9770 2 2 9)

摘　　要：目的　研究临床上用胰岛素作为载体通过受体介导对肝癌进行靶向治疗的可行性及其临床价值。方法　以A14 12 5I 标记胰岛素 ,取人肝癌细胞及癌旁组织 ,制备细胞膜 ,测定细胞膜胰岛素受体密度并评价其受体结合活性。结果　A14 12 5I 胰岛素比放射性为 (46 1± 1 2 )TBq/mmol,放射化学纯度 >98% ;人肝癌及癌旁组织细胞膜胰岛素受体Bmax分别为 (1 9± 0 6 )pmol/mg蛋白及(1 4± 0 6 )pmol/mg蛋白 ,高亲和力Kd(解离常数值 )值为 (2 1± 0 6 )nmol及 (2 2± 0 8)nmol,低亲和力Kd值为 (2 5± 8)nmol及 (2 7± 8)nmol。人肝癌细胞膜胰岛素受体表达高于癌旁组织 (最大结合值Bmaxt=0 0 32 ,P <0 0 5 )。结论　A14 12 5IObjectiveTo investigate the insulin receptor expression binding characteristics and it's clinical significance of 125 I insulin on human hepatocllular cancer cell membrane.MethodInsulin was radioiodinated using Ch T method, isolated and purified by polyacrylamide gel electryophresis. Human hepatocellular cancer and adjacent liver tissue cell membrane were extracted, receptor binding and bind ability of 125 I insulin and insulin receptor on hepatocellular cancer and adjacent hepatic cell membrane were studied. ResultsThe specific activity of 125 I insulin was 46 1±1 2 TBq/mmol,the radiochemical purity was over 98%. Insulin receptor content increased in hepatocellular carcinoma cells 〔Bmax=(1 9±0 6)?pmol/mg protein, the Kd of high and low affinity was 2 1±0 6?nmol and 25±8?nmol respectively〕 compared with carcinoma adjacent hepatic cells 〔Bmax=( 1 42± 0 57)?pmol/mg protein t =0 032 P <0 05, the Kd of high and low affinity was (2 2±0 8)?nmol and (27±8)?nmol respectively t =0 054, P >0 05〕.ConclusionHuman hepatocellular carcinoma cells overexpress insulin receptor. A14 125 I insulin has a specific high affinity with insulin receptor on HCC cells.

关 键 词：细胞膜 胰岛素受体 肝癌细胞 受体介导 靶向治疗载体 

分 类 号：R735.7[医药卫生—肿瘤]