压力对牛眼小梁细胞增殖和凋亡的影响  

Effect of pressure on proliferation and apoptosis of bovine trabecular meshwork

作　　者：薛蔚[1] 杜蜀华[1] 李勇[2] 杨业金[3] 

机构地区：[1]同济医科大学附属同济医院眼科,武汉430030 [2]协和医院外科 [3]同济医科大学分子生物学教研室

出　　处：《中华眼科杂志》2002年第6期340-343,共4页Chinese Journal of Ophthalmology

摘　　要：目的　探讨压力对牛眼小梁细胞的增殖周期及凋亡相关基因bcl 2和baxmRNA表达的影响。方法　体外培养牛眼小梁细胞。按照施加压力的大小分为实验组和对照组 ,对照组不施加压力 ,实验组分别给予 2 0、4 0、6 0及 80mmHg(1mmHg =0 133kPa)的压力 ,培养时间分别为 2 4及 4 8h。通过末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法、流式细胞仪和原位杂交技术 ,检测不同压力和持续时间下小梁细胞的增殖和凋亡指数 ,及在此条件下凋亡相关基因bcl 2和baxmRNA的表达。结果　≤ 4 0mmHg的压力持续 2 4h ,小梁细胞凋亡率与对照组比较差异无显著意义 (P >0 0 5 ) ;≥ 6 0mmHg的压力使小梁细胞凋亡率显著增高。 4 0mmHg的压力持续 2 4h后 ,细胞的凋亡指数虽未显著增高 ,但增殖指数已显著下降 ,bax基因表达有明显改变。≥ 4 0mmHg的压力持续 4 8h后 ,细胞的凋亡率、增殖指数及bcl 2家族基因表达量与对照组相比 ,差异均有显著意义 (均P <0 0 1)。结论　≥ 4 0mmHg的压力持续一段时间后 ,可通过影响bclObjective To investigate the effect of pressure on the expression of cell cycle and apoptosis related gene bcl 2 and bax mRNA by bovine trabecular meshwork cells. Method Twenty mm Hg , 40 mm Hg , 60 mm Hg and 80 mm Hg (1 mm Hg =0.133 kPa) pressure were given to the cultured trabecular meshwork cells respectively in the treatment groups. No pressure was given in the control groups. Each pressure sustained for 24 and 48 hours. Proliferation index, apoptosis index, bcl 2 and bax mRNA expression of trabecular meshwork cells were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling, flow cytometry and in situ hybridization. Results In 24 hour groups, there were no significant differences between the apoptosis index of control group and pressure ≤40 mm Hg groups. But in 40 mm Hg groups, the proliferative index decreased and bax mRNA expression increased significantly. The apoptosis index increased remarkably when the pressure was ≥60 mm Hg . In 48 hour groups, apoptosis index, proliferative index and bax mRNA expression had significant differences from that of the control groups when pressure was ≥40 mm Hg . Conclusion Pressure ≥40 mm Hg sustaining for a period of time can suppress trabecular meshwork cell proliferation and induce apoptosis through influencing bcl 2 family members.

关 键 词：小梁网 细胞分裂 脱噬作用 基因BCL-2 原癌基因 压力 牛眼 

分 类 号：R775[医药卫生—眼科]