利用RAPD技术分析实验用比格犬的遗传背景  

Analysis for Genetic Background of Laboratory Beagle Dog with Random Amplified Polymorphic DNA(RAPD)

作　　者：黄朝峰[1] 仲乃琴[1] 谭萍萍[1] 段子渊[1] 陈伟平[2] 刘运忠[2] 罗甜仪[2] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]广东省广州市医药工业研究所,广州510240

出　　处：《中国实验动物学报》2002年第2期65-68,共4页Acta Laboratorium Animalis Scientia Sinica

摘　　要：目的　运用RAPD技术对实验用比格犬 (Beagle)进行遗传背景分析。方法　筛选的 16个RAPD引物对 4 0个样品的分析 ,共得到 93个扩增条带。结果　所有样品的相似系数 80 4 %到 10 0 %间变动 ,平均相似系数为93 2 8% ,聚类分析得到了这些个体的同源树资料。Objective To analyse Random Amplified Polymorphic DNA(RAPD) for laboratory Beagle dogs so as to know their genetic background.Method\ 16 RAPD primers were selected and used to amplify 40 samples.Results\ In this study,93 bands were gained in agarose gel.The homology coefficient of all samples ranged from 80.4% to 100%,average at 93.28%.Assisted by DNAman software,the homology trees were produced by cluster analysis with all individuals and group samples.Conclusion\ Those genetic background materials can provide some technologic direction for Beagle dog breeding and applying in bio-medicine research.

关 键 词：RAPD技术 实验用比格犬 遗传背景 

分 类 号：Q95－331[生物学—动物学] R－332[医药卫生]