白念珠菌胞壁β-葡聚糖诱导人外周血单个核细胞产生IL-6和IL-8的研究  

The effect of β-glucan of cell wall from Candida albicans on interleukin-6 and interleukin-8 production by human peripheral blood mononuclear cells in vitro

作　　者：李岷[1] 陈青[2] 孙君江[2] 沈永年[1] 吕桂霞[1] 刘维达[1] 吴绍熙[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,江苏南京210042 [2]南京市第一医院,江苏南京210002

出　　处：《临床皮肤科杂志》2002年第6期349-350,共2页Journal of Clinical Dermatology

摘　　要：不同浓度的白念珠菌胞壁碱溶性和不溶性β-葡聚糖、白念珠菌菌悬液与人外周血单个核细胞共孵育24h,阳性对照为IL-6、8诱导剂脂多糖,并设不加刺激剂的空白对照,酶联免疫法检测上清液中白介素-6、8(IL-6、8)的含量。结果表明,3种浓度不溶性β-葡聚糖均能诱导产生IL-6、8,而碱溶性β-葡聚糖3种浓度均未能诱生这两种因子。5×107、5×105CFU/mL白念珠菌菌悬液能诱导产生IL-6和IL-8,5×103CFU/mL则未能。提示此胞壁成分的抗原性及其对临床的意义。The levels of interleukin 6(IL 6) and IL 8 in the supernatant of human peripheral blood mononuclear cells(PBMC) stimulated by different concentration of alkali soluble and insoluble β glucan of cell wall from Candida albicans were detected by ELISA to investigate whether these two kinds of glucan could be capable of inducing PBMC cells to secret IL 6 and IL 8. The results showed IL 6 and IL 8 could be induced by 3 concentrations of insoluble β glucan, but alkali soluble β glucan could not induce these two cytokines at any concentration.

关 键 词：白念珠菌 Β-葡聚糖 白细胞介素-6 白细胞介素-8 外周血单个核细胞 

分 类 号：R379.4[医药卫生—病原生物学]