恶性胸水肿瘤细胞的分离、纯化及其临床意义  

Tumor cell isolation and purification from malignant pleural effusion and its clinical significance

作　　者：马吉勇[1] 庞玉瑛[1] 王明丽[2] 朱钟鸣[1] 

机构地区：[1]安徽医科大学第一附属医院呼吸内科,合肥230022 [2]安徽医科大学病原微生物教研室,合肥230032

出　　处：《安徽医科大学学报》2002年第2期132-134,共3页Acta Universitatis Medicinalis Anhui

摘　　要：目的　探讨恶性胸水肿瘤细胞的分离、纯化方法学以及临床意义。方法　在 2 0份胸水标本中 ,同一份标本分别按离心沉渣法、密度梯度离心法和不连续密度梯度离心法 3种方法分为 3组检测 :细胞病理学 (Ⅰ组 )、单个核细胞 (Ⅱ组 )、肿瘤细胞 (Ⅲ组 )。分别计算各组阳性率 ,各组间率的比较行配对资料 χ2 检验。结果　Ⅰ组 :肿瘤细胞阳性率为45 %(9/ 2 0 ) ;Ⅱ组 :肿瘤细胞阳性率为 6 5 %(13/ 2 0 ) ;Ⅲ组 :肿瘤细胞阳性率为 2 0 %(4 / 2 0 )。Ⅰ组与Ⅱ组、Ⅰ组与Ⅲ组组间比较差异均无显著性 (P >0 0 5 ) ,Ⅱ组与Ⅲ组比较差异有显著性 (P <0 0 1)。结论　采用密度梯度离心法和不连续密度梯度离心法可以从恶性胸水中分离出肿瘤细胞 ,并有助于鉴别恶性胸水中肿瘤细胞的细胞分化和细胞起源。Objective To investigate the methodology of tumor cells isolation from malignant pleural effusion and its clinical significance. Methods Twenty patients with malignant pleural effusion were tested. Every sample was randomly divided into cytology group(Ⅰ), mononuclear cells group(Ⅱ) and tumor cells group(Ⅲ) by means of centrifugation smear assay, density gradient centrifugation and discontinuous density gradient one. Positive rate of tumor cells was calculated and compared with each other. Difference was evaluated by matched data chi-square test in three groups. Results These was no statistical difference between group Ⅰ and group Ⅱ as group Ⅰ and group Ⅲ(P>0.05). And there is statistical defference between group Ⅱ and group Ⅲ(P<0.01). Conclusion Tumor cell can be separated from malignant pleural effusion by discontinuous density gradient centrifugation and it is beneficial to the diagnosis of tumor cells cytogenesis.

关 键 词：恶性胸水 肿瘤细胞 分离 纯化 临床意义 梯密度离心法 

分 类 号：R730.4[医药卫生—肿瘤] R561.3[医药卫生—临床医学]