刺五加叶皂甙诱发肝癌细胞凋亡的研究  

Effects of acanthophopanax senticosus on cell cycle of line of hepatocellular carcinoma cells

作　　者：叶红军[1] 邹兵[1] 杜意平[1] 

机构地区：[1]北京大学深圳医院,广东深圳518036

出　　处：《临床肝胆病杂志》2002年第3期162-163,共2页Journal of Clinical Hepatology

摘　　要：探讨刺五加 (ASS)叶皂甙诱导体外培养肝癌细胞凋亡的作用。流式细胞仪检测ASS对HepG2 肝癌细胞的细胞周期动力学和凋亡诱导率的影响 ,并用维甲酸 (RA)作为对照药物。结果ASS和RA均作用于细胞周期的S期 ,抑制肝癌细胞的DNA合成。ASS还减少G2 M期细胞数量 ,抑制细胞有丝分裂 ,诱发细胞凋亡率显著高于RA的作用 ,上述效应与其作用时间和剂量有关。ASS可促进体外培养肝癌细胞凋亡 ,有必要进一步探索ASS对肝癌的治疗价值。To investigate the effects of Acanthophopanax Senticosus(ASS) on inducing apoptosis of hepatocellular carcinoma(HCC) cells. The effects of ASS on cell cycle and apoptosis rate of HCC cells were observed with flow cytometry assays, and Retinoic acid(RA) was used as the control drug. The results showed both ASS and RA might inhibit DNA synthesis of HCC cells, and apoptosis rate induced by ASS is significantly higher than that induced by RA. It is suggested that ASS could induce apoptosis of HCC cells. it is necessary to study further the therapeutic value of ASS on HCC.

关 键 词：刺五加 细胞凋亡 刺五加叶皂甙 细胞周期动力学 肝癌细胞 ASS 

分 类 号：R735.7[医药卫生—肿瘤]