显微操作仪快速分离癌细胞并提取微量RNA的方法  

Rapid isolation of cancer cells from tumor tissue by micromanuiplator and extraction of tiny amount of RNA

作　　者：江培洲[1] 沈新明[1] 黄华[1] 石益民[1] 姚开泰[1] 

机构地区：[1]第一军医大学病理学教研室,广东广州510515

出　　处：《第一军医大学学报》2002年第6期551-553,共3页Journal of First Military Medical University

基　　金：广东省自然科学基金项目(013050);广东省科技计划项目(A1080201)

摘　　要：目的建立一种快速分离、纯化肿瘤组织癌细胞并提取微量RNA的方法。方法将肿瘤组织冰冻切片,快速染色,在镜下用显微操作仪从切片上分离癌巢,收集癌细胞,提取RNA并鉴定。结果冰冻切片染色清楚,癌巢分离准确,成功抽提到高质量的RNA。结论显微操作仪可以代替激光捕获显微切割仪进行特定细胞的分离,该方法可用来从少量组织的特定细胞中抽提RNA。Objective To establish a rapid method for isolating and purifying cancer cells from tumor tissue and for RNA extraction from tiny amount of the cells thus obtained. Methods Frozen sections of the tumor tissues were prepared followed by rapid staining. Clusters of the cancer cells were isolated from the sections by micromanipulation technique and purified for extracting intact RNA that was subsequently assayed. Results Clear vision was achieved by the staining of the sections. The cancer cell clusters were precisely isolated from which high-quality intact RNA was obtained as demonstrated by reverse transcriptase-PCR. Conclusion Micromanuiplation can be effectively used in stead of laser capture microdissection to isolate and purify targeted cells from tiny amount of tissue samples, therefore making RNA extraction possible in this context.

关 键 词：显微操作仪 快速分离 癌细胞 提取 微量RNA 显微切片术 显微切割 

分 类 号：R730.2[医药卫生—肿瘤]