层粘连蛋白受体与胆管癌细胞不同转移性亚群的关系研究  

STUDY ON RELATION OF LAMININ RECEPTOR AND DIFFERENT BILE DUCT CARCINOMA METASTATIC CELL STRAINS

作　　者：陈长宏[1] 王曙光[1] 李大江[1] 刘利[1] 

机构地区：[1]第三军医大学西南医院

出　　处：《解放军医学杂志》2002年第6期509-511,T003,共4页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39870 830 )

摘　　要：探讨胆管癌细胞不同转移性亚群层粘连蛋白受体的表达差异。以腹膜浸润筛选模型筛选胆管癌细胞高、低转移性亚群 ,体外侵袭实验验证筛选结果 ,流式细胞仪比较两亚群间层粘连蛋白受体的表达差异。结果显示 ,本研究成功地以腹膜浸润筛选模型筛选出胆管癌细胞高、低转移性亚群 ,体外侵袭实验进一步验证了两亚群间存在着侵袭能力上的显著差异。高、低转移性亚群层粘连蛋白受体的阳性细胞百分数分别为 (36 36± 9 2 2 ) %和 (13 17±3 4 3) % ,两者间差异显著 (P <0 0 5 )。提示层粘连蛋白受体的表达与体外胆管癌细胞的不同转移倾向密切相关。To investigate the expression of laminin receptor in different bile duct carcinoma cell strains, a peritoneum infiltration screening model was established to screen out the different metastatic cell strains of bile duct carcinoma. Invasive experiments in vitro were adopted to validate the results. The difference of LNR expression was compared both in the high and low cell strains by flow cytometry analysis. The results showed that by using the peritoneum infiltration screening model, high and low metastatic QBC939 cell strains were successfully screened out. Invasive experiments in vitro also proved that the invasive ability of high cell strain was stronger than that of the low one. The positive rate of LNR in high strains (36 36±9 22)%was higher than that in the low ones(13 17±3 43)%, and the difference was significant( P <0 05).It can be concluded that laminin receptor may be related with the metastatic process of bile duct carcinoma.

关 键 词：层粘连蛋白受体 胆管细胞 转移性亚群 研究 肿瘤转移 

分 类 号：R735.8[医药卫生—肿瘤]