大鼠局灶性脑缺血后脑内HO-2蛋白的表达  

The Neuronal Expression of Heme Oxygenase Protein During Focal Cerebral Ischemia in Rats

作　　者：陈衔城 符荣 任惠民 季耀东 宋后燕[3] 殷信道 任军[3] 

机构地区：[1]复旦大学附属华山医院神经外科,200040 [2]复旦大学附属华山医院神经病学研究所,200040 [3]复旦大学医学院分子遗传实验室,200040 [4]复旦大学附属华山医院放射科,200040

出　　处：《中国临床神经科学》2002年第2期136-139,共4页Chinese Journal of Clinical Neurosciences

基　　金：复旦大学优秀博士学位论文培育资助项目创新基金 (CQF15180 1)

摘　　要：目的 :研究局灶性脑缺血时脑内血红素氧合酶 2 (HO 2 )的表达。方法 :在大鼠大脑中动脉阻塞致脑缺血的不同时间点应用免疫组化、病理学及图像分析检测HO 2的表达。结果 :正常大鼠及实验大鼠的梗死侧与非梗死侧皮质、丘脑基底节区和海马齿状回均有HO 2阳性神经元。梗死灶内无表达。梗死后各时间段 ,HO 2阳性神经元的数量无明显变化 (P >0 0 5) ,而图像分析显示1 2～ 2 4h时达最高峰 (P <0 0 1 )。结论 :脑缺血时脑内神经元有HO 2蛋白阳性表达 。Aim:To study the expression of heme oxygenase(HO) 2 protein during focal cerebral ischemia in rats.Methods:The expression of heme oxygenase(HO) 2 protein in the brain of rats during focal cerebral ischemia were investigated by using immunohistochemistry,pathological study and computer image analysis.Results:HO 2 expression was observed in the bilateral cerebral tissues including infarction cortex border area,thalamus,basilar ganglion and hippocampus.With time going on,the numbers of HO 2 positive neurons are equal( P >0 05),but the expression of HO 2 has changed,peaked at 12～24 h ( P <0 01) Conclusion:There is an expression of HO 2 protein in brain tissue during cerebral ischemia,but its mechanism of brain protection remains unclear.

关 键 词：大鼠 局灶性脑缺血 脑内HO-2蛋白 一氧化碳 血红素氧合酶-2 免疫组化 

分 类 号：R743.31[医药卫生—神经病学与精神病学]