FasL基因诱导胶质瘤细胞凋亡和抑制增殖作用的研究  

Effect of FasL Gene on Induction of Cell Apoptosis and Proliferation of Human Glioma Cells

作　　者：郑宇[1] 浦佩玉[1] 王春艳[1] 

机构地区：[1]天津医科大学总医院神经外科,300052

出　　处：《中国临床神经科学》2002年第2期140-142,149,共4页Chinese Journal of Clinical Neurosciences

摘　　要：目的 :探讨FasL基因诱导胶质瘤细胞凋亡和抑制增殖的作用。方法 :脂质体介导FasL基因转染TJ90 5胶质瘤细胞系 ,原位杂交 ,FasL免疫组化鉴定转染成功 ,应用MTT法、Ki 67免疫组化检测TJ90 5细胞的增殖变化 ,TUNEL法检测细胞凋亡。结果 :FasL基因转染成功后 ,肿瘤细胞内FasL表达增加 ,细胞增殖率降低 ,而凋亡细胞数增多。结论Aim:To study the effect of FasL gene on the apoptosis and proliferation of human glioma cells in vitro.Methods:FasL gene transfection into human gliomablastoma cell line was mediated by lipofectamine,the FasL gene expression was examined with in situ hybridization and immunohistochemical staining.Using MTT assay,Ki 67 immunohistochemical staining and TUNEL method to study the surviving rate,proliferating activity and cell apotosis respectively.Results:FasL expression of TJ905 cells was enhanced after gene transfection,the cell surviving rate was lowered, and so as proliferating activity,while the amount of cells undergoing apoptosis was increased.Conclusion:Apoptosis induced by FasL gene may become a promising approach for the treatment of gliomas.

关 键 词：FASL基因 诱导 胶质瘤 细胞凋亡 抑制增殖作用 颅内肿瘤 

分 类 号：R739.41[医药卫生—肿瘤]