食管癌旁上皮及原位癌中p53蛋白的表达及意义  

The expression of p53 protein in human esophageal precancerous lesions and carcinoma in situ and its significance

作　　者：陶仪声[1] 宗永生[2] 

机构地区：[1]蚌埠医学院病理教研室 [2]广东省中山医科大学病理教研室,510089

出　　处：《临床肿瘤学杂志》2002年第3期168-169,共2页Chinese Clinical Oncology

基　　金：美国中华医学基金会及安徽省教育厅科研基金资助课题(97JL14 8)

摘　　要：目的 :探讨食管癌旁正常上皮、增生上皮、不典型增生上皮及原位癌中抑癌基因p5 3蛋白的异常表达情况及其意义。方法 :用HE染色确定上皮类型。用免疫组化LSAB法检测各种癌旁上皮及原位癌中p5 3蛋白的表达。结果 :正常粘膜上皮中无p5 3蛋白的积聚。增生上皮p5 3蛋白阳性率为 5 5 %,但阳性细胞少 ,染色浅。不典型增生上皮p5 3蛋白阳性率为79 %,且阳性细胞逐渐增多 ,染色加深 ;原位癌中p5 3蛋白阳性率为 98%。三者之间经 χ2 检验差异有显著性 (P <0 0 1)。结论 :p5 3蛋白积聚在食管上皮癌变前即已存在 ,在原位癌中积聚更加显著。可以作为上皮恶性转化的客观指标之一。Objective:To study the overexpression of p53 protein in human basal cell hyperplasia to dysplasia to carcinoma in situ and their significance.Methods:189 specimens of esophageal precancerous lesions and carcinomas in situ were tested for p53 by using immunohistochemical technic.Results:Positive stain was not observed in the dividing basal cell of the normal epithelium.From basal cell hyperplasia to dysplasia to carcinoma in situ,the p53 immunostain-positive cell increased in number,positive-rates were 55%?79% and 98% (P<0.01).Conclusion:p53 protein accumulation occurs early in esophageal precancerous lesions.It may be an indicator of epithelium malignancy in human esophageal.

关 键 词：食管癌 原位癌 癌旁上皮 P53基因 蛋白表达 临床意义 

分 类 号：R735.1[医药卫生—肿瘤]