APA微囊包裹对牛肾上腺嗜铬细胞分泌儿茶酚胺和亮氨酸脑啡肽的影响  

The Affection of APA Microcapsulation on Catecholamine and Leucine-enkephalin Secretion from the Bovine Adrenal Medullary Chromaffin Cells

作　　者：郭水龙[1] 崔忻[1] 罗芸[1] 薛毅珑[1] 

机构地区：[1]解放军总医院细胞生物室,北京100853

出　　处：《标记免疫分析与临床》2002年第2期80-82,共3页Labeled Immunoassays and Clinical Medicine

摘　　要：分析海藻酸钠 -多聚赖氨酸 -海藻酸钠 (APA)微囊包裹对牛肾上腺嗜铬细胞 (BCCs)分泌儿茶酚胺 (CA)和亮氨酸脑啡肽 (L -EK)的影响。用APA微囊包裹原代BCCs ,分别用高效液相色谱 -电化学法和放射免疫法检测囊化与非囊化的BCCs分泌CA和L -EK的能力。与非囊化BCCs相比 ,囊化BCCs在单位时间内分泌肾上腺素 (E)和去甲肾上腺素 (NE)的能力并没有大的改变 ,而多巴胺 (DA)和L -EK的分泌量则在部分时间点有明显下降。APA微囊对牛肾上腺嗜铬细胞分泌E和NE并无显著的影响 ,而对DA和L -EK的分泌有一定影响 ,这种影响仅在包裹后短期内发生 。The affection of alginate-polylysine-alginate(APA) microcapsulation on catecholamine(CA) and leucine-enkephalin (L-EK) secretion from bovine adrenal medullary chromaffin cells was analysed. Encapsulating BCCs with the APA microcapsulation, the secretion of CA and L-EK in encapsulated BCCs was detected by high-performance liquid chromatography-electrochemical assay and radioimmunoassay, respectively. There is little difference between the encapsulated BCCs and the non-encapsulated BCCs in the secretion of epinephrine(E) and noradrenaline(NE) but the secretion of dopamine(DA) and L-EK in several points decline. The studies indicated that APA microcapsulation of BCCs didn′t affect the secretion of AD and NE, but did the secretion DA and L-EK in forepart.

关 键 词：AP微囊包裹 肾上腺嗜铬细胞 镇痛物质 儿茶酚胺 亮氨酸脑啡肽 

分 类 号：R741.05[医药卫生—神经病学与精神病学]