超氧化物歧化酶对骨骼肌I/R后粘附分子表达的影响  

Effect of superoxide dismutase on adhesion molecules expression in skeletal muscle ischemia/reperfusion injury in rats

作　　者：吕松岑[1] 王新涛[1] 文立臣 韩竹[1] 陶天遵[1] 关德宏[1] 

机构地区：[1]哈尔滨医科大学附属第二医院手外科,150086 [2]大庆市第五医院骨科

出　　处：《中华医学杂志》2002年第12期840-843,共4页National Medical Journal of China

基　　金：黑龙江省自然基金资助 (D982 7)

摘　　要：目的　探讨超氧化物歧化酶 (SOD)在骨骼肌缺血再灌注 (I/R)后对白细胞表面粘附分子CD11b/CD18和骨骼肌血管中细胞间粘附分子 (ICAM) 1表达的影响。方法　建立大鼠后肢缺血再灌注模型 ,10 2只大鼠随机分为假手术组 (Ⅰ组 ,n =6 )、缺血组 (Ⅱ组 ,n =6 )、缺血再灌注组 (Ⅲ组 ,n =30 )、生理盐水组 (Ⅳ组 ,n =30 )、SOD组 (Ⅴ组 ,n =30 )。Ⅱ组在缺血 4h末 ,Ⅲ、Ⅳ、Ⅴ组分别于再灌注 1、2、4、8、12h末 ,测定血浆中的丙二醛 (MDA)、骨骼肌组织中的髓过氧化物酶 (MPO) ,白细胞上粘附分子CD11b/CD18和骨骼肌血管中ICAM 1的表达情况及各组的组织学改变。结果　Ⅲ组各时相点MDA、MPO、CD11b/CD18、ICAM 1的表达与Ⅰ组比较 ,差异有非常显著意义 ,骨骼肌组织损伤也随再灌注时间的延长而逐渐加重 ,Ⅴ组中则这种变化明显抑制 (P <0 0 5 )。Ⅳ组和Ⅲ组之间比较 ,差异无显著意义。结论　粘附分子CD11b/CD18和ICAM 1与骨骼肌I/R损伤密切相关 ,SOD不仅可以清除I/R时产生的氧自由基 ,还可以抑制粘附分子的表达 ,减少白细胞在骨骼肌组织中的浸润 。Objective To study the effect of SOD on the expression of CD11b/CD18 and ICAM 1 after skeletal muscle ischemia / reperfusion injury in rats Methods Establish the rat model of hind limb ischemia / reperfusion One hundred and two rats were divided into 5 groups: sham operation group (GroupⅠ n =6), ischemia group (GroupⅡ n =6), ischemia / reperfusion injury group (GroupⅢ n =30), normal saline treatment group (GroupⅣ n =30), superoxide dismutase treatment group (GroupⅤ n =30) Flow cytometric analysis and immunohistochemistry were used to assess the expression of CD11b/CD18 on the leukocytes, ICAM 1 in skeletal muscle at the end of ischemia and 1h, 2h, 4h,8h,12h after reperfusion The changes of MDA in the plasma,MPO in the skeletal muscle and the tissue morphology were studied too Results In groupⅢ,the expression of CD11b/CD18, ICAM 1,MDA,MPO were increased evidently The longer time of reperfusion,the more serious the skeletal muscle′s injury was In groupⅤ, the changes were ameliorated as compared with groupⅢ( P <0 05), groupⅣ has no difference with groupⅢ Conclusion These data indicate that the expression of CD11b/CD18 and ICAM 1 were significantly correlated with skeletal muscle ischemia/reperfusion injury SOD can inhibit the expression of free radicals and the adhesion molecules,consequently skeletal muscle ischemia/reperfusion injury is prevented

关 键 词：再灌注损伤 超氧化物歧化酶 细胞粘着分子 骨骼肌缺血 

分 类 号：R363[医药卫生—病理学]