大鼠CAG证病结合模型胃粘膜病理研究  

Pathological Changes of Gastric Mucosal in Rat Models of Combined CAG Syndrome and Disease

作　　者：陈小野[1] 邹世洁[1] 佟彤[1] 樊雅莉[1] 张宇鹏[1] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700

出　　处：《中医药学刊》2002年第3期292-294,共3页Study Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目 (39370 86 4)

摘　　要：目的 :建立新的大鼠慢性萎缩性胃炎 (CAG)证病结合模型 ,并观察其实验 35周的胃粘膜病理改变。方法 :雌性大鼠 ,分为对照组、CAG组、脾虚CAG组、肝郁CAG组、肾虚CAG组。CAG模型采用脱氧胆酸钠和阿斯匹林水溶液交替饮用加免疫损伤法 ,脾虚证模型采用耗气破气加饥饱失常法 ,肝郁证模型采用夹尾加肾上腺素注射法 ,肾虚证模型采用MTU溶液饮用法。总造模期 35周。造模结束后光镜观察胃粘膜病理改变。结果 :CAG模型各组胃粘膜有明显的萎缩性改变 ,而炎证和纤维化程度相对较轻。组间的差异主要表现在胃粘膜萎缩的程度上 ,以脾虚CAG组和肾虚CAG组最为严重。肝郁CAG组胃粘膜萎缩程度较轻。结论 :本实验在完善CAG证病结合模型方面作了尝试 ,并以之初步观察了证、病和证病结合在胃粘膜病理上的反映。Objective:To establish a new chronic atrophic gastritis(CAG)model combined syndrome with disease in rat,Then to observe the pathological change of gastric mucosal in 35 weeks after experiment.Methods:Female Rats were assigned to 5 groups,including CAG,deficiency of the spleen of CAG,stagnation of the liver-qi of CAG,deficiency of the kidney of CAG model groups and control group.Models were respectively induced with sodium deoxycholate and asprine add to damage the immune system,relieve stagnation of qi with drastic drugs add to abnormal ingestion irregularity of food,tuck rat's tail and injection of addrenalin,drinking MTU.After 35 weeks we observed the pathological changes of the gastric mucosal by optics microscope.Results:It all can be obviously observed atrophic gastric mucosal in CAG models.The trait is the degree of inflammation and fibrosis was not severe.The great diversity in groups is the difference of atrophic degree of gastric mucosal,which is lighter in stagnation of the liver-qi models than in the deficiency of the spleen models and deficiency of the Kidney models.conclusions:We try to perfect the methods to make the models in this experiment,then observed pathological changes of gastric mucosal in syndrome,disease,and combined syndrome with disease.

关 键 词：大鼠 CAG证病结合模型 胃粘膜病理 萎缩性胃炎 

分 类 号：R573.320.2[医药卫生—消化系统]