细胞因子联合激活人骨髓和外周血免疫细胞的比较研究  

Comparative Study on Activated Immunocytes of Human Bone Marrow and Peripheral Blood by Cytokines

作　　者：黄文荣[1] 张伯龙[1] 靳海杰[1] 高春记[1] 达万明[1] 汪月增[1] 

机构地区：[1]中国人民解放军总医院血液科,北京100853

出　　处：《中国实验血液学杂志》2002年第3期222-225,共4页Journal of Experimental Hematology

摘　　要：在本研究中比较了某些细胞因子在体外联合激活骨髓和外周血免疫细胞后二者免疫细胞数量、形态、细胞化学染色、免疫表型和细胞毒变化的差异。在体外加入IFN γ ,rIL 1,rIL 2和McAb CD3分别激活培养骨髓和外周血单个核细胞 ,培养过程中观察两组免疫细胞增殖数量及形态的变化 ,培养前后两组取样进行细胞化学染色和免疫表型检测 ,用MTT法检测培养后两组的细胞毒情况。研究结果发现 ,两组的细胞数量在激活培养后均较培养前明显增加 ,但外周血组增加倍数更多 (P <0 .0 5 ) ;细胞化学染色可见两组培养以后髓过氧化物酶积分均较培养前减少 ,过碘酸雪夫染色见两组含较多粗颗粒的淋巴样细胞明显较培养前多 ;两组CD3+,CD5 6 +和CD38+细胞较培养前明显增加 (P <0 .0 5 ) ,但两组增殖无明显差别 ;骨髓组培养后CD3+CD5 6 +细胞无明显增加 ,而外周血组培养后则增加显著 (P <0 .0 5 ) ;两组激活培养后细胞的细胞毒无明显差异。结论 :IFN γ ,rIL 1,rIL 2和CD3单抗联合激活骨髓和外周血单个核细胞可使二者细胞数量和细胞毒明显增加 ,在临床上可利用激活的不同来源的细胞因子诱导的杀伤细胞进行细胞免疫治疗。To study immunophenotype and cytotoxicity of the immunocytes in bone marrow and peripheral blood after activation by combined cytokines, mononuclear cells(MNC) of bone marrow and peripheral blood were activated by IFN γ , IL 1, IL 2 and McAb CD3 in vitro. The cell amount and morphology during culture were observed. Cytochemical staining and immunophenotype analysis were done before and after culture in two groups of MNC. Cytotoxicity was tested by MTT method. The results showed that the cell number of two groups increased obviously in culture(P<0.05), while the peripheral blood mononuclear cells increased more markedly(P<0.05). The cytochemial staining showed POX decrease, but PAS increase in two groups. The positive ratios of CD3 +, CD56 + and CD38 + cells in two groups increased obviously after culture(P<0.05), but there was no significant difference between those two groups. CD3 + CD56 + cells increased obviously in peripheral blood mononuclear cells activated by cytokines(P< 0.05 ), but CD3 + CD56 + cells did not increase in bone marrow mononuclear cells. There was no significant difference between two groups′ cytotoxicity. It was concluded that IFN γ, IL 1, IL 2 and McAb CD3 increased cell number and cytotoxicity of both bone marrow and peripheral blood mononuclear cells that can be used in cell immunotherapy.

关 键 词：细胞因子 骨髓细胞 外周血细胞 免疫细胞 免疫表型 细胞毒 肿瘤治疗 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤]