幽门螺杆菌UreB和HspA DNA疫苗的构建及免疫评价  

Construction and immunological evaluation of DNA vaccine of UreB and HspA of Helicobacter pylori

作　　者：刘秀丽[1] 李淑琴[1] 刘纯杰[1] 陶好霞[1] 李勣[1] 张兆山[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071

出　　处：《生物技术通讯》2002年第3期161-163,共3页Letters in Biotechnology

摘　　要：本研究选择幽门螺杆菌尿素酶B和热休克蛋白A为候选抗原,通过PCR扩增基因,克隆至真核表达质粒pCD-NA3.1(-)His-Myc上,构建成DNA疫苗。通过小鼠免疫效果的评价,获得两株具有免疫源性DNA疫苗。To testify the effect of DNA vaccine of Helicobacter pylori,the Urease B and Heat-shock protein A were chose as candidate antigens.Amplified by PCR,the UreB and HspA gene were cloned into the plasmid pCDNA3.1(-)His-Myc.Immunized through muscle injection,the Bal B/c mice produced the antisera against UreB or HspA.

关 键 词：幽门螺杆菌 UREB HSPA DNA疫苗 构建 免疫评价 尿素酶B 热休克蛋白A 候选抗原 

分 类 号：R573.1[医药卫生—消化系统] R392.2[医药卫生—内科学]