鸡瘦蛋白受体(OBR)基因外显子9单核苷酸多态性分析  

Single Nucleotide Polymorphism Analysis in Chicken Leptin Receptor Exon 9

作　　者：顾志良[1] 赵建国[1] 李辉[1] 孟和[1] 王启贵[1] 王清华[1] 朱大海[2] 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]哈尔滨工业大学生命科学与工程系,哈尔滨150001

出　　处：《遗传》2002年第3期259-262,共4页Hereditas（Beijing)

基　　金："国家自然科学基金"(项目编号 30 0 70 5 5 2 );教育部"高等学校骨干教师资助计划"资助

摘　　要：瘦蛋白受体属于Ⅰ类细胞因子超家族受体 ,其在瘦蛋白的信号转导中起着关键的作用。本研究根据瘦蛋白受体基因外显子 9的序列设计引物 ,用PCR -SSCP的方法对高脂系 (fatnessline,FL)、低脂系 (leannessline ,LL)、北京油鸡、白耳鸡、石歧杂、矮小黄鸡、小型黄鸡、惠阳胡须鸡、隐性白羽鸡和海兰蛋鸡等品种 (系 )进行了单核苷酸多态性分析 ,并检测到了多态性。对两种纯合子片段克隆和测序 ,结果表明在编码区的 116 7位碱基发生了突变 ,由C突变为A ,但是编码的氨基酸并没有发生改变。经独立性检验 ,基因型频率和基因频率分布与品种有关 ,北京油鸡的AA基因型频率显著高于其他品种 ;高脂系中A基因频率显著高于低脂系。Leptin receptor is a type I cytokine super family member and plays an important role in leptin functioning signal transduction.This study was designed to investigate the single nucleotide polymorphism (SNP) of OBR gene in various breeds,including Fatness Line (FL),Leanness Line (LL),Beijing Youji,Baierji,Shiqiza,Dwarf Yellow Chickens,Mini Yellow Chickens,Huiyang Huxuji,Recessive White Chickens and Hyline Layer.The primers for exon 9 in OBR gene were designed from the database of chicken genomic sequence and the SNPs were detected by PCR-SSCP method.One SNP (C/A at 1167 in cds) was found among individuals within all breeds.However,the amino acid was not changed because it was a silence mutantion.The result of population genetics analyses showed that the frequency of AA genotype in Beijing Youji was significantly higher than that in other lines.Also,the frequency of A allele in FL was significantly higher than that in LL.

关 键 词：鸡 瘦蛋白受体 外显子 单核苷酸多态性 OBR基因 

分 类 号：S831.1[农业科学—畜牧学]