双价抗虫基因叶绿体共转化植株抗虫性及其后代表型分析  

The Insect Resistance of OC and Bt Transplastomic Plant and the Phenotype of Their Progenies

作　　者：苏宁[1] 孙萌[2] 杨波[1] 孟昆[1] 刘春英[1] 倪丕冲[1] 沈桂芳[1] 

机构地区：[1]中国农业科学院生物技术研究所叶绿体基因工程实验室,北京100081 [2]浙江大学生命科学院,杭州310027

出　　处：《遗传》2002年第3期288-292,共5页Hereditas（Beijing)

基　　金：国家自然科学基金资助 ( 395 70 36 1)

摘　　要：利用基因枪法将含有水稻巯基蛋白酶抑制剂 (Oryzacystatin ,OC)基因烟草叶绿体表达载体和含有苏云金芽孢杆菌晶体毒蛋白基因 (BtcryIAc)烟草叶绿体表达载体 ,共转化烟草叶绿体 ,获得壮观霉素抗性植株。转基因植株抗棉铃虫试验表明 ,转双价抗虫基因植株比转单一抗虫基因植株具有更强的杀虫活性。转基因植株后代Southern检测及其遗传学分析试验证明 ,双价抗虫基因可以稳定地遗传给后代 ,且表现为叶绿体特有的母系遗传规律。The Bt gene and OC gene were cotransformed to tobacco chloroplast with particle bombardment method and spectinomycin resistance tobacco seedlings were obtained.Bioassays showed that the transgenic tobacco containing both genes had enhanced toxicity to the larvae of cotton bollworm (helicoverpa zea) by comparison with the plants containing only Bt or OC gene.Southern-blotting analysis and genetic analysis of progenies showed that the Bt and OC gene expressed and was inherited maternally to the progenies.

关 键 词：双价抗虫基因 叶绿体 共转化植株 抗虫性 后代 表型分析 OC基因 BT基因 

分 类 号：Q943.2[生物学—植物学]