P16蛋白和生精细胞凋亡对热压和11酸睾酮诱导的恒河猴无精子症和少精子症中的作用  

ROLE OF P16 PROTEIN AND GERM CELL APOPTOSIS IN RHESUS MONKEY AZOOSPERMIA OR OLIGOZOOSPERMIA INDUCED BY HEAT STRESS OR TESTOSTERONE UNDECANOATE

作　　者：周新昌[1] 胡召元[1] 李因传[1] 邹如金[2] 刘以训[1] 

机构地区：[1]中国科学院动物研究所生殖生物学国家重点实验室,北京100080 [2]中国科学院昆明动物研究所,昆明650223

出　　处：《动物学报》2002年第3期363-367,共5页ACTA ZOOLOGICA SINICA

基　　金：国家"973" (G19990 5 5 90 1)计划;攀登预选项目;中国科学院知识创新工程方向项目 (KSCX 2 SW 2 0 1);国家自然科学基金 ( 39870 36 8)资助项目

摘　　要：探讨了P16蛋白和生精细胞凋亡在热压和 11酸睾酮诱导恒河猴无精子症和少精子症中作用间的关系。3′末端标记分析 (TUNEL)结果显示热应激和超生理剂量睾酮能够诱导生精细胞出现凋亡信号 ,它分别于处理后第 5天和第 30天达到最强。免疫组化结果显示 ,热压或TU主要诱导精原细胞和其它生精细胞以及Sertoli细胞P16的表达。P16蛋白的表达在生精细胞凋亡晚期 ,即隐睾手术第 10天或注射TU第 6 0天后迅速升高并维持高表达 ,该蛋白在生精细胞凋亡晚期可能通过抑制精原细胞的有丝分裂 ,扰乱正常的精子发生。上述结果提示 ,在热压或 11酸睾酮诱导的早期精母细胞和精子细胞的凋亡和在晚期对精原细胞有丝分裂的抑制 ,二者共同作用导致热压或TU诱导的恒河猴无精子症和少精子症。We investigated the functional relationship between the role of P16 protein and germ cell apoptosis in heat stress or TU induced azoospermia or oligo zoospermia.The TUNEL result showed that the apoptotic signals after treatment increased and the strongest apoptotic signals appeared in the germ cells of the cryptorchid testes on day 5 or in the TU treated monkey testes on day 30.The immunohistochemical result showed that both heat stress and TU induced expression of P16 protein mainly in spermatogonia,and also in other type of germ cells and sertoli cell at the later stage of germ cell apoptosis,namely on day 10 after operation or on day 60 after TU injection respectively, suggesting that P16 protein may inhibit the spermatogonia mitosis at the later stage of the germ cell apoptosis.The above results indicated that both heat stress and TU treatment were capable of inducing germ cell apoptosis at the early stage on one hand,and inhibiting spermatogonia mitosis at the later stage in the testis on the other hand,which might result in azoospermia or oligozoospermia.

关 键 词：P16蛋白 生精细胞凋亡 热压 11酸睾酮 恒河猴 无精子症 少精子症 作用 

分 类 号：R-332[医药卫生]