日本鬼鲉毒腺cDNA表达文库的构建和初步分析  

The Construction of cDNA Expression Library from the Venom of Inimicus japonicus

作　　者：姜孝玉[1] 涂洪斌[1] 陈慧萍[1] 卫剑文[1] 杨文利[1] 吴文言[1] 徐安龙[1] 

机构地区：[1]中山大学生命科学学院

出　　处：《生物化学与生物物理进展》2002年第3期424-428,共5页Progress In Biochemistry and Biophysics

基　　金：国家高新技术海洋领域 863项目 (819 0 6 0 6);国家自然科学基金重点项目 (6993 5 0 2 0 ) .~~

摘　　要：以海洋生物日本鬼鲉毒腺为材料 ,应用SMARTTM cDNALibraryConstruction技术 ,构建以真核表达载体pcDNA3 0为基础的日本鬼鲉毒腺cDNA表达文库 .通过对文库克隆的序列测定和初步生物信息学分析 ,获得 94个日本鬼鲉毒腺新表达序列标签 (ESTs) ,其中已确定全长cDNA的克隆有 35个 ,包括溶细胞素基因、类短链神经毒素蛋白、C型凝集素、巨噬细胞迁移抑制因子、致病相关基因等 ,这些基因在日本鬼鲉中绝大多数为首次发现 .日本鬼鲉毒腺cDNA表达文库的成功构建 ,为深入研究日本鬼鲉毒腺的组分及其分子作用机理奠定了基础 .A cDNA library of the venom of Inimicus japonicus was constructed. The cDNA was cloned into eukaryotical expression plasmid pcDNA3 0. SMART TM protocol was used for cDNA library construction and bioinformatics analysis was carried out. 94 novel EST clones were obtained from 150 sequences in the library, of which there were 35 full length clones, including cytolysin genes, short neurotoxin gene, C type lectin gene, macrophage migration inhibitory factor gene and so on. Most of those genes were reported for the first time in Inimicus japonicus . Further studies on those genes and large scales of sequencing in the library are going on, which may be helpful to make clear the components of Inimicus japonicus venom and understand the function of those proteins at molecular level.

关 键 词：日本鬼Yuo 毒腺 CDNA表达文库 刺毒鱼类 

分 类 号：R996.3[医药卫生—毒理学] Q785[医药卫生—药学]