辐射诱导基因LRIGx的细胞周期特异性表达及编码产物同源性分析  

Cell Cycle Dependent Expression of Radiation-induced Gene LRIGx and The Homologous Comparison of Its Encoding Product

作　　者：周平坤[1] 隋建丽[1] 耿煜[1] Odile RIGAUD 吴德昌 

机构地区：[1]北京放射医学研究所,北京100850 [2]CEA/DSV/DRR,Laboratoire de Radiobiologie Cellulaire

出　　处：《中国生物化学与分子生物学报》2002年第3期272-276,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家高技术研究发展计划 ( 86 3计划 ;No .2 0 0 1AA2 2 12 71);国家自然科学基金 (No .39870 2 14 );"十五"军队医学杰出中青年基金 (No .0 1J0 0 6 )

摘　　要：低剂量辐射诱导表达新基因LRIGx被克隆 .Northern印迹杂交结果表明 ,在 0 2Gyγ射线照射后 2～ 4h ,人A5 4 9细胞中该基因mRNA表达水平显著上调 .当照射剂量增加到 2Gy时 ,其诱导表达水平明显低于 0 2Gy照射 .通过细胞周期同步化 ,观察到LRIGx基因表达高峰在G2 M期 .同源性比较和功能保守域分析结果显示 ,该基因编码产物与DNA修复和重组蛋白RAD5 4、ERCC 6 ,染色质重构和转录调节功能蛋白SWI2 SNF2等有同源性 ,其N端具有与染色质重构、基因转录调控和DNA修复有关的 3个功能结构域 ,即CHROMO。A novel low dose radiation induced expression gene named LRIGx was cloned. Northern analysis revealed that the mRNA level of LRIGx was significantly up regulated in A549 cells at 2～4 hours after 0 2 Gy of γ ray exposure. However, the induced level of LRIGx mRNA by 2 0 Gy was not so significant as that by 0 2 Gy low dose exposure. Using synchronized cells, an expression peak of LRIGx mRNA was observed in G 2/M phase cells. The results of homologous comparison indicated that the N terminal of LRIGx encoding product contained some identical domains with DNA repair and recombination protein RAD54, ERCC 6, chromosome remodeling and transcription regulating protein SWI2/SNF2. These domains included CHROMO, SNF2N and helicase C.

关 键 词：辐射诱导基因 LRIGx 细胞周期 特异性表达 编码产物 同源性分析 DNA修复 

分 类 号：R811.5[医药卫生—放射医学] Q345[医药卫生—临床医学]