用组织微阵列技术分析鼻咽癌变多阶段组织细胞周期蛋白D_1过表达  

Tissue Microarray Analysis of Cyclin D_1 Gene Overexpression in The Multistage Carcinogenesis of Nasopharyngeal Carcinoma

作　　者：宋鑫[1] 赵晓荣[1] 王一[2] 周建华[3] 关新元[4] 曹亚[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078 [2]第二军医大学东方肝胆外科医院,上海200438 [3]中南大学湘雅医院病理学教研室,长沙410078 [4]香港大学临床肿瘤学系

出　　处：《生物化学与生物物理进展》2002年第3期385-389,共5页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展规划 (973 )项目 (G19980 5 12 0 1);国家自然科学基金 (3 0 0 0 0 0 87和 3 0 10 0 0 0 5 );国家自然科学基金杰出青年基金 (3 95 2 5 0 2 2 )资助~~

摘　　要：为了探讨细胞周期蛋白D1(CyclinD1)在鼻咽癌变多阶段病变组织中的蛋白质表达与鼻咽癌变的关系 ,应用组织微阵列 (tissuemicroarray)技术 ,采用免疫组化方法检测CyclinD1在鼻咽癌旁粘膜上皮单纯性增生 /化生 (simplehyperplasia/metaplasia ,SH/SM )、异型增生 /化生 (atypicalhyperplasia/metaplasia,AH/AM )和鼻咽癌 (nasopharyngealcarcinoma,NPC)的蛋白质表达 ,阳性表达率分别为 30 0 % (3/ 10 )、 90 0 % (18/ 2 0 )和6 2 9% (39/ 6 2 ) ,其中在异型增生 /化生中呈“一过性增高” .表明CyclinD1高表达可能为鼻咽癌发生过程中的早期事件 ;异型增生 /化生可能是鼻咽癌变的重要“关卡” .In order to investigate the relation between Cyclin D-1 protein expression in multistage tissue of pathological changes during the nasopharyngeal carcinogenesis and nasopharyngeal carcinoma (NPC), the simple hyperplasia/metaplasia, atypical hyperplasia/metaplasia in the nasopharyngeal paracarcinoma mucous epithelium and NPC were studied for Cyclin D-1 expression with immunohistochemical streptavidin-peroxidase (SP) method by tissue array technique. The positive rates were 30.0% (3/10), 90.0% (18/20) and 62.9% (39/62) respectively, among which hightening 'instantaneously' in the atypical hyperplasia/metaplasia appeared. It is shown high Cyclin D-1 expression may be an early incident in the course of nasopharyngeal carcinogenesis, and that atypical hyperplasia/metaplasia probably be an important 'toll-gate'.

关 键 词：组织微阵列技术 分析 鼻咽癌变 多阶段组织细胞周期蛋白D1 过表达 

分 类 号：R739.63[医药卫生—肿瘤]