短暂性缺血对小脑皮质影响的组织化学研究  

HISTOCHEMICAL STUDY OF THE AFFECTION OF TEMPORARY CEREBRAL ISCHEMIA ON THE CEREBELLAR CORTEX

作　　者：李仁[1] 顾远清[1] 陈显斌[1] 李昊[2] 刘爱民 熊希凯[3] 吴刚[1] 

机构地区：[1]湖北民族学院医学院解剖,组胚学教研室,恩施445000 [2]湖北中医学院附属医院,武汉430061 [3]同济医科大学解剖学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2001年第1期37-40,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：湖北省高校自然科学重点科研项目 (96 A0 6 8)

摘　　要：为了探讨全脑短暂性缺血对小脑皮质蒲肯野细胞的影响 ,实验用组织化学方法对家兔全脑缺血 5分钟 (B组 )、 10分钟 (C组 )及缺血再灌 (D、 E组 )后蒲肯野细胞的酶组织化学变化进行了观察。结果显示 ,缺血 10分钟及缺血再灌后蒲肯野细胞的 SDH、Mg2 + - ATP活性及 PAS反应均降低 (P<0 .0 5 ) ,L DH增高 (P<0 .0 1)。结果提示家兔全脑缺血In order to explore the affection of temporary cerebral ischemia on the purkinje cells of the cerebellary cortex. Using histochemical method, enzymehistochemical changes was observed in the purkinje cells of the rabbit after ischemia of 5 minutes (group B)? 10 minutes (group C) and reperfusion (group D?E). The results showed that after 10 minutes of ischemia and reperfusion. The activites of SDH?Mg 2+ ATP?PAS were reduced ( P <0 05), While the activites of LDH wre increased ( P <0 01). The results suggested that the injury of 10 minutes ischemia and reperfusion may damage the activites of enzymes associated with energic metabolism in cerebellar cortex of purkinje cells.

关 键 词：短暂性缺血 小脑皮质 影响 组织化学研究 脑缺血 

分 类 号：R743.31[医药卫生—神经病学与精神病学]