转录因子E2F1和PCNA在分离、培养的人视网膜色素上皮细胞中的表达及意义  

EXPRESSION OF TRANSCRIPTION FACTOR E2F1 AND PCNA IN CULTURED HUMAN RETINAL PIGMENT EPITHELIAL CELLS

作　　者：李晓青[1] 曾水清[1] 徐莉莉[1] 吕源淑[1] 

机构地区：[1]同济医科大学协和医院眼科,武汉430022

出　　处：《中国组织化学与细胞化学杂志》2001年第2期146-149,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：为探讨转录因子 E2 F1及增殖细胞核抗原 (PCN A)在增生性玻璃体视网膜病变 (PVR)中的作用 ,采用 SABC免疫细胞化学法检测转录因子 E2 F1和 PCNA在无血清组及 2 0 %血清组培养的人视网膜色素上皮细胞 (RPE)中的表达。结果显示在无血清组中 ,转录因子 E2 F1表达的阳性率为 2 .4%± 0 .2 5 % ,而未见 PCNA表达 ;在 2 0 %血清组中 ,E2 F1及 PCNA的表达阳性率分别为 2 9.1%± 0 .71%和 48.6 %± 1.13%。说明 E2 F1和 PCNA蛋白的表达取决于人 RPE细胞的生长状态 ,在增殖活跃的细胞中表达增高 ;转录因子 E2 F1调控人 RPE细胞中 PCNA蛋白的表达。提示转录因子 E2 F1调控人 RPE细胞的细胞周期进展 ,在In order to provide evidence for the effects of E2F1 and PCNA in proliferative vitreoretinopathy (PVR), the expression of transcription factor E2F1 and PCNA in cultured human RPE cells of serum deprived group and serum contained one were detected by using immunocytochemical SABC technique. The positive staining rate for E2F1 was 2 4%±0 25% while the expression of PCNA was rat secn; the ones for E2F1 and PCNA were 29 1%±0 71% and 48 6%±1 13% respectively. These findings indicated that the expression of E2F1 and PCNA may be associated with the growth activity of cultured human RPE cells and E2F1 regulated the expression of PCNA protein. It suggested that transcription factor E2F1 regulate the cell cycle progress of human RPE cells and it also play an important role in the development of PVR.\;

关 键 词：转录因子E2F1 PCNA 分离 培养 表达 增殖细胞核抗原 视网膜色素上皮细胞 免疫组织化学 人 PVR 

分 类 号：R774.1[医药卫生—眼科] Q436[医药卫生—临床医学]