cyclin D1,cyclin E在乳腺良、恶性病变中的表达及其与p16,p21waf1及Rb蛋白的关系  

EXPRESSION OF CYCLIN D1, CYCLIN E IN BENIGN AND CANCEROUS BREAST TISSUES AND THEIR RELATIONSHIPS WITH P16, P21 waf1 AND RETINOBLASTOMA TUMOR SUPPRESSOR PROTEIN

作　　者：吴强[1] 张瑰红[1] 饶慧蓉[1] 杨枫[1] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032

出　　处：《中国组织化学与细胞化学杂志》2001年第3期307-310,357,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：安徽省教委自然科学基金资助课题 (No.98jl0 6 1)

摘　　要：为探讨 cyclin D1,cyclin E在乳腺癌发生发展中的作用及其与细胞周期调控相关基因蛋白的关系 ,采用免疫组化检测 17例非增生乳腺导管上皮、 19例不同程度增生的导管上皮及 5 9例乳腺癌中 cyclin D1,cyclin E,p16 ,p2 1waf1及 Rb基因蛋白的表达。结果显示 :1.非增生乳腺导管上皮除 1例 cyclin E过表达外均无 cyclin D1和 cyclin E的过表达。乳腺癌的 cyclin D1和 cyclin E的过表达率均明显高于良性乳腺组织 (P<0 .0 5 )。 2 .乳腺癌 cyclin D1过表达与淋巴结转移呈正相关 (P<0 .0 5 ) ,瘤体直径大于 5 cm者 cyclin E过表达呈增加趋势 ,但差异无显著性意义。 3.Cyclin D1和 cyclin E的过表达呈正相关 (P<0 .0 5 )。 4.从非增生乳腺导管上皮到增生直至乳腺癌 ,p16 ,p2 1与 cyclin D1,cyclin E含量的比值逐渐递减 ,而 p2 1含量高于 cyclin D1的乳腺癌体积小、淋巴结转移率低 (P<0 .0 5 )。 p2 1阳性率与 cyclin D1过表达呈正相关 (P<0 .0 1) ,也随 cyclin E的过表达呈上升趋势。 Rb基因蛋白的强表达与 cyclin D1过表达呈正相关 (P<0 .0 1)。结果表明 :CyclinD1和 cyclin E蛋白过表达频发于乳腺癌早期 ,它们可能与 p16、 p2 1waf1、 pIn order to explore the effects of G1 S cell cycle regulating proteins on mammary carcinogenesis and progression, cyclin D1, cyclin E, p16, p 21 waf1 and pRb expressions were examined immunohistochemically in 36 benign breast tissues, including 17 cases without and 19 with epithelial hyperplasia, and 59 breast carcinomas. No overexpression for cyclin D1 and cyclin E was detected in duct epithelia without hyperplasia except one case with cyclin E overexpression. The positive rates of cyclin D1 and cyclin E in breast cancer were significantly higher than those in benign tissues( P <0 05). There was a positive correlation between cyclin D1 overexpression and lymph node involvement( P <0 05), and there was a trend for tumors larger than 5cm to overexpress cyclin E more frequently. A positive correlation was evident between overexpression of cyclin D1 and cyclin E( P <0 05). The ratios of p16 and p21 to cyclin D1 and cyclin E contents decreased from normal breast epithelia, hyperplasia to carcinomas. Tumors with higher p21 than cyclin D1 content were smaller in size and had lower frequency of node involvement( P <0 05). p21 positivity was correlated with cyclin D1 overexpression( P <0 01),and there was a trend to ward more cyclin E overexpression with the increasing expression of p21. Strong expression of pRb was positively associated with cyclin D1 overexpression ( P <0 01). The results suggest that cyclin D1 and cyclin E overexpressions occur in the early stage of breast carcinoma, which might play an important role in the transformation and development of breast cancer cooperating with genes of p16, p21waf1 and Rb.

关 键 词：cyclinD1 CYCLINE 恶性病变 P16 p21wafl RB蛋白 乳腺肿瘤 癌基因 抑癌基因 基因表达 细胞周期 免疫组织化学 良性病变 

分 类 号：R737.9[医药卫生—肿瘤]