伪狂犬病病毒上海株gE和gI基因的克隆及缺失转移载体的构建  

Cloning, Construction of gE Gene and gI Gene Partial DeletionMutant of Pseudorabies Virus SH Strain

作　　者：姜焱[1] 侯玉峰[2] 陈德胜[1] 陈溥言[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京出入境检验检疫局,江苏南京210001

出　　处：《中国病毒学》2002年第2期149-152,共4页Virologica Sinica

基　　金：上海市农委重点攻关课题 ( 98 0 5 7)

摘　　要：根据已发表的伪狂犬病病毒 (PseudorabiesVirus,PRV)的 gI和 gE 基因序列 ,设计两对引物用PCR方法扩增出PRV SH gI和 gE 基因 ,将其克隆入 pUC18载体中 ,获得了缺失部分gI基因的转移载体质粒 ,命名为 pgEI。According to the Genbank open sequence,two pairs of primer were designed in order to amplify gE gene and partial gI gene of pseudorabies virus Shanghai strain,respectively.The gE gene and partial gI gene were obtained by polymerase chain reaction(PCR) ,subsequently cloned into pGEM-T vector.Then both of them were inserted into pUC18 vector.The recombinant plasmid pgEI deleted part of gI gene was constructed.

关 键 词：GE基因 基因克隆 动物病毒 伪狂犬病病毒 上海株 GI基因 缺失转移载体 构建 

分 类 号：S852.65[农业科学—基础兽医学]