抑郁症大鼠海马和顶叶皮质神经元NGF含量及其mRNA的表达  

Changes of NGF level and NGF mRNA expression in hippocampal and parietal cortical neurons in experimental depression rats

作　　者：王雪琦[1] 由振东[1] 赵小林[1] 王勇姿[1] 路长林[1] 

机构地区：[1]第二军医大学基础医学部神经生物学教研室,上海200433

出　　处：《第二军医大学学报》2002年第6期590-592,I006,共4页Academic Journal of Second Military Medical University

基　　金：全军医学科研"十五"计划青年基金资助项目(0 1Q0 94) ;全军医学科研"十五"计划基金重点资助项目 (0 1Z0 65 ) ;国家自然科学基金重点资助项目 (3 993 0 0 80 ) .

摘　　要：目的 :观察实验性抑郁症大鼠脑内神经生长因子含量及其 m RNA表达的变化。 方法 :采用长期不可预见性中等强度应激造成大鼠抑郁症模型。运用 openfield法观察大鼠行为学变化 ,运用免疫组化和原位杂交法观察神经元 NGF含量及其m RNA表达的变化。 结果 :与对照组相比 ,抑郁组大鼠海马和顶叶皮质神经元 NGF免疫阳性颗粒数目减少 ,着色浅淡 ;NGFm RNA杂交阳性信号减少。 结论 :实验性抑郁症大鼠海马和顶叶皮质神经元 NGF含量下降 ,NGF m RNA表达水平降低。Objective: To observe the changes of NGF level and NGF mRNA expression in experimental depressive model of rats. Methods: The depression model of rats was established by chronic unpredictable mild stress. Openfield test was performed to detect the behavior of rats. Immunohistochemistry and in situ hybridization was used to observe the changes of NGF level and NGF mRNA expression. Results: Compared to the control group, the NGF like immunoreactivity and signals of NGF mRNA in situ hybridization of depressive group were significantly decreased. Conclusion: The levels of NGF and NGF mRNA decrease significantly in experimental depressive model of rats.

关 键 词：抑郁症 大鼠 海马 顶叶皮质 神经元 NGF 含量 mRNA 神经生长因子 

分 类 号：R749.4[医药卫生—神经病学与精神病学]