应激时大鼠垂体前叶血管紧张素原基因表达增强  

Stress induced expression of the angiotensinogen gene in rat anterior pituitary

作　　者：张素珍[1] 徐永[2] 万瑜[3] 杨钢[3] 唐志佼[4] 胡建民[5] 

机构地区：[1]广东医学院病理生理教研室,广东湛江524023 [2]广东医学院生理教研室,广东湛江524023 [3]武汉大学医学院生理教研室,湖北武汉430071 [4]武汉大学医学院病理教研室,湖北武汉430071 [5]武汉大学医学院实验中心,湖北武汉430071

出　　处：《中国病理生理杂志》2002年第5期540-542,T002,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :观察应激时大鼠垂体前叶血管紧张素原mRNA表达及血管紧张素Ⅱ含量的变化。方法 :用原位杂交技术及放免分析法检测应激大鼠垂体前叶血管紧张素原 (ANG)mRNA的表达及血管紧张素Ⅱ (AngⅡ )含量。结果 :应激时垂体前叶ANGmRNA表达及AngⅡ含量均显著增高 ,且ANGmRNA表达增高率超过了AngⅡ含量的增高率。结论 :应激时垂体前叶肾素 -血管紧张素系统 (RAS)被显著激活 ,进一步支持局部RAS参与了应激反应的观点。AIM: To investigate the expression of angiotensinogen (ANG)mRNA and the changes of AngⅡ content in rat anterior pituitary during acute stress.METHODS: The expression of ANG mRNA was determined using in situ hybridization technique, and the AngⅡ content were determined by RIA.RESULTS: The ANG mRNA expression and AngⅡ content were significantly increased, and ANG mRNA was increased more significantly than AngⅡ content. CONCLUSION: The local renin-angiotensin system (RAS) in anterior pituitary is obviously activated during stress, which strongly supports the view that local RAS contributes to stress reaction.

关 键 词：应激 大鼠 垂体前叶血管紧张素原 基因表达 原位杂交 

分 类 号：R335.1[医药卫生—人体生理学]