金银花绿原酸酶法提取新工艺研究  

Study on extraction process of chlorogenic acid in Flos Lonicerae by enzymic treatment

作　　者：刘佳佳 赵国玲[1] 章晓骅[1] 王晖[1] 

机构地区：[1]中南大学制药工程系,湖南长沙410083

出　　处：《中成药》2002年第6期416-418,共3页Chinese Traditional Patent Medicine

摘　　要：目的 :优化金银花中绿原酸的提取工艺。方法 :将金银花乙醇回流前 ,用纤维素酶和果胶酶分别或联合处理 ,探讨酶的用量、处理时间、处理温度及酶的联合作用对金银花提取物得率和绿原酸得率的影响。结果 :纤维素酶处理能显著提高金银花提取物得率和绿原酸得率 (8.15 g/ 10 0g) ,酶处理最适温度为 4 0℃～ 5 0℃。纤维素酶和果胶酶的联合处理对绿原酸得率影响不明显 ,但能显著提高提取物得率。采用该工艺绿原酸得率最高可达到 8.32 %。结论Objective: To optimize the extraction process of chlorogenic acid in flos lonicerae.Methods: The flos lonicerae was treated by means of cellulase and/or pectolase before extracted by ethanol reflux, studying the enzyme dosage, treatment time, treatment temperature how to influence the percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction.Results: The percentage of extraction of flos lonicerae and the amount of chlorogenic acid in extrction could be obviously increased by the cellulase treatment, 8.15g chlorogenic acid could be extracted from 100g flos lonicerae. The optimum temperature of treatment was 40℃～50℃; The percentage of extraction was higher by the combined treatment of cellulase and pectilase than by cellulase only, but the amount of chlogenic acid was not obviously different between these two methods.Conclusion: The amount of chlorogenic acid in extrction could be increased by enzymic treatment.

关 键 词：金银花 绿原酸 酶处理 提取工艺 

分 类 号：R284.2[医药卫生—中药学]