同源盒基因在胶质瘤细胞中的表达  

Expression of Hox gene in Rat C6Glioma Cell Line

作　　者：杨朝华[1] 田宇[2] 卜丽莎[2] 赵丛海[2] 任同 

机构地区：[1]广西壮族自治区北海市人民医院神经外科 [2]吉林大学中日联谊医院神经外科,长春市130031

出　　处：《中国肿瘤临床》2002年第6期418-420,共3页Chinese Journal of Clinical Oncology

基　　金：本文课题受国家自然科学基金(39900152);吉林省科委科研基金资助(990574-3)

摘　　要：目的:观察胶质瘤细胞C6中同源盒基因(Hox基因组)的表达,及苯乙酸(PA)对Hox基因组表达的影响。方法:体外培养C6细胞并用PA诱导分化,提取细胞RNA。用Hox基因三对简并引物P1、P2和P3进行逆转录PCR(RT-PCR)。通过计算机图像分析,Hox基因组mRNA的表达水平用Hox基因(组)/β-肌动蛋白(β-actin)灰度比值表示。应用PA前后基因表达的差异用t检验分析。结果:在胶质瘤细胞C6中,P1、P2和P3组Hox基因表达分别为0.8817±0.0731(n=16),0.6825±0.0987(n=9),0.8608±0.0881(n=16);应用PA后,分别为:0.8765±0.0667(n=8),0.8386±0.0811(n=14),0.8937±0.0598(n=11)。应用PA后,P2组Hox基因表达显著增强,与用药前比较,差异显著(P<0.001)。结论:在胶质瘤C6细胞中,PA对P2组Hox基因mRNA水平的表达有明显的上调作用。PA的作用机理与调节胶质瘤细胞转录过程可能有关。Objective:To study the expression of Hox gene in rat C6glioma cell line and the effect of PA on Hox gene expression.Methods:C6cells were cultured in vitro and induced differentiation by PA,then the total cellular RNA was extracted.In the presence of Hox gene degenerate primers(P1,P2,P3),RT-PCR was performed.The level of Hox gene mRNA expression was shown as Hox gene /β-actin according to the computer image analysis and the difference between C6cells and PA treated C6cells was analysed by t-test.Results:In C6cells,the ex-pression levels of Hox genes in P1,P2and P3were0.8817±0.0731(n=16),0.6825±0.0987(n=9)and0.8608±0.0881(n=16)respectively.After PA treatment,the expression levels were0.8765±0.0667(n=8),0.8386±0.0811(n=14)and0.8937±0.0598(n=11)respectively.The expression of P2was significantly strengthened after PA treatment (P<0.001).Conclusion:The ex pression of Hox gene mRNA in P2group is up-regulated by PA in C6cells.The mechanism of PA action is correlated with the transcription process in glioma cells.

关 键 词：表达 苯乙酸 胶质瘤 同源盒基因 

分 类 号：R730.2[医药卫生—肿瘤]