B系急性淋巴细胞性白血病患儿诱导疗程前、疗程中及缓解期外周血中T-淋巴细胞DNA增殖能力的研究  

The Study of DNA Synthesis in T-lymphocytes of Children with B-Acute Lymphoblastic Leukemia

作　　者：顾绍庆[1] 曹巧云[1] 张希云[2] 彭惠兰[3] 孔向蓉[4] 

机构地区：[1]江苏大学附属人民医院儿科,江苏镇江212001 [2]江苏大学医学院临床二系,江苏镇江212001 [3]苏州大学附属儿童医院血液科,江苏苏州215000 [4]苏州大学放射医学系,江苏苏州215000

出　　处：《江苏大学学报（医学版）》2002年第2期123-125,共3页Journal of Jiangsu University：Medicine Edition

摘　　要：目的 :观察诱导疗程前、疗程中及缓解期 3个时期内B系急淋患儿外周血T淋巴细胞DNA体外合成能力的变化情况。方法 :应用3 H TDR掺入法。结果 :在B系急淋未经治疗的情况下 ,T淋巴细胞的DNA体外合成受到抑制。在诱导疗程后期 ,T淋巴细胞的DNA体外合成能力仍然底下。在缓解期 ,T淋巴细胞的DNA体外合成能力明显增强 ,甚至略高于正常水平。结论 :B系急淋患儿在自然状态下T淋巴细胞转化水平显著低下 ;在诱导疗程后期 ,T淋巴细胞转化水平仍然低下 ;在治疗后缓解期T淋巴细胞转化水平恢复正常 ,甚至出现反跳现象。同时 ,我们认为无限增殖的肿瘤细胞和大剂量的化疗药物是影响患儿T细胞功能的主要因素。Objective:To observe the different levels of different rates of DNA synthesis in T lymphocytes of children with B-Acute Lymphoblastic Leukemia(B-ALL) in three stages of pre-,mid-chemotherapy and remission.Methods: 3H-TDR incorporations were used in this study.Results:The rate of DNA synthesis was lower than that of the control group before chemotherapy. When the patients were being treated to induce remission, the rate of DNA synthesis was still lower than that of the control group.When remission, the rate of DNA synthesis increased evidently.Conclusions:From the above we know that patients' cellular immunity was bad before treatment, and which was still bad when being treated to induce remission, when the patients were in remission, their cellular immunity also restored. Furthermore, we think that the great numbers of leukemic cells and large dose antitumor drugs are two reasons of the low level of patients' cellular immunity.

关 键 词：B-急性淋巴细胞性白血病 T淋巴细胞 DNA 

分 类 号：R733.71[医药卫生—肿瘤]