富硒玉米油不皂化物成分的分离与测定  

Analysis on Unsaponifiables in Corn Oil Rich in Selenium

作　　者：苏青云[1] 严煤[1] 郭祖铭 李和[3] 李佩文[3] 

机构地区：[1]华中农业大学理学院,武汉430070 [2]湖北省宣恩县人民政府,宣恩445500 [3]北京师范大学,北京100875

出　　处：《华中农业大学学报》2002年第3期281-284,共4页Journal of Huazhong Agricultural University

摘　　要：用制备薄层层析法将富硒玉米油的不皂化物分成 7个部分 ,然后用气相色谱法分别测定甾醇、4 -甲基甾醇和三萜醇的组成。分离、鉴定了Δ7-豆甾烯醇乙酸酯 (Δ7-stigmastenolacetate)、β -谷甾醇乙酸酯 (β -sitosterolacetate)、豆甾醇乙酸酯 (stigmasterolacetate)、Δ5-燕麦甾醇乙酸酯 (Δ5-avenasterolacetate)等The unsaponifiables from mentioned corn oil rich in selenium were separated by designed TLC of silica gel G into 7 fractions. The sterol,4-methylsterol and triterpene alcohol were analyzed quantitatively by GC. Insterol fraction, Δ 7 -stigmastenol acetate,β-sitosterol acetate,stigmasterol acetate and Δ 5 -avenasterol acetate were separated by TLC of silica gel G impregnated with silver nitrate.They were identified by melting point determination,optical rotation and gas chromotography.

关 键 词：富硒玉米油 不皂化物 成分 分离 测定 气相色谱分析法 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]