碳纤维微电极差示脉冲伏安法大量抗坏血酸存在下测定痕量多巴胺  

Study on Determining of Dopamine at Carbon Fiber Microelectrode by Different Pulse Voltammetry

作　　者：刘灵芳[1] 冶保献[2] 李风菊[2] 王业民[2] 

机构地区：[1]信息工程大学测绘学院,河南郑州450052 [2]郑州大学化学系,河南郑州450052

出　　处：《测绘学院学报》2002年第2期154-156,共3页Journal of Institute of Surveying and Mapping

基　　金：河南省杰出青年基金资助课题 (990 2 4)

摘　　要：报导了利用一种特定的电化学方法将自制的碳纤维微柱电极活化 ,可以有效地增强其对多巴胺 (DA)的电化学响应 ,且使抗坏血酸 (AA)和DA的氧化峰电位差达 2 6 0mV ,有效地避免了AA对DA测定的干扰。利用高灵敏的差示脉冲吸附伏安技术使DA的检测限达 4 .0× 10 -8mol/L ,峰电流与DA浓度在 6 .0× 10 -8～ 1.0× 10 -6mol/L范围内呈良好线性关系 ,并且 ,实验过程无需除氧 ,可以满足在体检测DA的各种条件 ,可望用于活体检测。此外 。In this paper, report a new method for determination of trace dopamine at the pretence of high concentration of ascorbic acid. The carbon fiber microelectrode is electro-actived with a fixed electrochemical method. The actived microelectrode possess high ability to distinguish dopamine and ascorbic acid with ΔE p=260 mV. At selective condition with different pulse voltammetry, the peak currents are linear relationship with DA concentration in the range of 6.0×10 -8 ～1.0×10 -6 mol/L with detect limit 4.0×10 -8 mol/L. All experimental process is performed without deaeration, which is an indispensable condition in vivo study.

关 键 词：碳纤维 微电极 差示脉冲伏安法 多巴胺 抗坏血酸 

分 类 号：O629.4[理学—有机化学]