MTT法体外药敏试验指导下胃肠癌的预见性化疗  

Predictive Chemotherapy of Gastric-intestinal Cancer Directed by MTT Assay in Vitro

作　　者：匡久树[1] 蒋芹[1] 黄小明[1] 葛旭明[1] 李小民[1] 张为民[1] 谢晓东[1] 刘淑华[1] 

机构地区：[1]连云港市第一人民医院肿瘤科,江苏连云港222002

出　　处：《肿瘤防治杂志》2002年第3期288-290,共3页China Journal of Cancer Prevention and Treatment

摘　　要：目的 :研究MTT法体外药敏试验指导下胃肠癌预见性化疗的临床意义。方法 :取 2 7例新鲜胃肠癌标本进行体外MTT法药物敏感试验 (MTT试验组 ) ,2 7例同期胃肠癌患者作经验对照组。试验组按药敏试验结果化疗 ,耐药者按临床经验化疗 ;对照组按临床经验化疗。结果 :2 7例药敏试验完成实验 ,药敏阳性者胃癌 8例 ,肠癌 5例 ;阴性者胃癌 7例 ,肠癌 7例。临床化疗结果 :试验组中对药物敏感者化疗有效率 76 9% ,耐药者中有效率 14 3% ,经统计学处理差异有显著性 ,χ2 =10 7,P <0 0 1;试验组总有效率为 4 4 4 %。经验对照组化疗后有效率为 2 5 9% ,经统计学处理两组疗效差异有显著性 ,χ2=9 5 ,P <0 0 1。MTT实验结果与临床化疗结果的符合率为 81 4 8% (10 + 12 / 2 7)。结论 :MTT法检测癌细胞对抗癌药或敏感试验可以预见胃肠癌的化疗。Objective To study the predictive value in vitro using MTT assay for chemotherapy to gastric intestinal cancer patients.Methods Chemosensitivity testing of fresh tumour cells in 27 patients with gastric intestinal cancer was conducted by the MTT assay.The MTT sensitive group were given chemotherapy according to the drug sensitivity tesults in MTT assay;The MTT resistant group were given chemotherapy according to clinical experience.The control group were given chemotherapy according to clinical experience.Results The MTT assay could be performed for the 27 patients.The response rate in sensitive group and resistant group respectively were 76 9%(10/13) and 14 3%(2/14), χ 2=10 7, P <0 01, There was significant statistical difference.The overall response rate in MTT assay group was 44 4%(12/27).The response rate in control group was 25 9%.χ 2=9 5, P <0 01.There was significant difference between the two group.Between vitro and vivo the overall coincident rate was 81 48%(10+12/27).Conclusions The MTT assay can predict chemotherapy for gastric intestinal cancer.

关 键 词：比色法 胃肠肿瘤 药物疗法 药物耐受性 

分 类 号：R735[医药卫生—肿瘤] R730.53[医药卫生—临床医学]