外照射对体外培养的小细胞肺癌细胞系的总RNA及多药耐药基因的影响  

Influence on the Total RNA and MDR1 Gene of NCI-H446 Cell Line by Fructionated Radiation

作　　者：段蕴铀[1] 宋鹏[1] 聂青[1] 黄友章[1] 周丽君[1] 王洪武[1] 胡圆[1] 

机构地区：[1]海军总医院呼吸科,北京100037

出　　处：《海军总医院学报》2002年第2期65-67,共3页Journal of Naval General Hospital of PLA

摘　　要：目的　观察分次外照射对NCI H4 4 6小细胞肺癌细胞系的总RNA及其MDR1基因的影响。方法　采用GWGP80型远距离6 0 Co治疗机对进入指数生长期的NCI H4 4 6小细胞肺癌细胞系进行分次外照射 ,每次 2Gy ,总剂量为 5 0Gy。用Trizol分别提取未照射和已完成全部外照射剂量的NCI H4 4 6细胞系的总RNA。通过RT PCR ,琼脂胶电泳 ,应用Gelbase电脑软件计算电泳条带的平均光密度值 (光密度值愈大 ,表示产物愈多 ) ,求两组细胞的MDR1DNA与其 β actinDNA的平均光密度值的比来确定两组细胞MDR1mRNA表达的高低。 结果在相同细胞数和相同体积的条件下 ,未照射组和照射组细胞总RNA的浓度分别为 2 5 9μg ml和 16 6 μg ml;未照射组细胞其MDR1DNA β actinDNA为 1 0 78,照射组为 1 338。结论　对NCI H4 4 6小细胞肺癌细胞系进行外照射可抑制其总RNA的生物合成 ;Objective The aim of this study was to investigate the effect of fractionated radiation treatment, given in a regimen similar to those administered clinically, on the total RNA and MDR1 gene of NCI H446 small cell lung cancer cell line.Methods Exponentially growing NCI H446 cells were exposed to 50 Gy radiation administered in 25 fractions, at 2 Gy per fractions. The total RNA of the cells that were irradiated and unirradiated was isolated from homogenate samples by the acid guanidine thiocyanate phenol chloroform method. The expression of MDR1 gene was assessed by qualitative RT PCR assays. A portion of each sample from RT PCR assays was electrophoresed on a 1% agar gel, strained with ethidium bromide and visualized under UV light. We used Gelbase software to calculate the average OD value of every electrophoresis trip and acquired the average OD value's ratio of MDR1DNA and its β actin DNA of the two kinds of cells. The larger ratio indicates the increase of MDR1DNA's expression.Results To the unirradiated cells, the concentration of the total RNA was 25.9 μg/ml and the ratio of the average value of electrophoresis trip from MDR1DNA/β actin DNA was 1.078; But to the irradiated cells, this two numerical values were 16.6 μg/ml and 1.338 respectively.Conclusion Radiation treatment to the NCI H446 small cell lung cancer cell line can inhibit the synthesis of its total RNA and enhance the expression of its MDR1 gene at the same time.\;

关 键 词：细胞系 小细胞肺癌 外照射 总RNA MDR1mRNA 

分 类 号：R734.2[医药卫生—肿瘤]