PTSD样情感行为异常大鼠脑组织ATP酶活性改变  

ALTERATION OF ATPase ACTIVITIES IN HIPPOCAMPI OF RATS WITH PTSD-LIKE BEHAVIOR

作　　者：肖凯[1] 王庆松[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所四室,重庆400042

出　　处：《西南国防医药》2002年第3期204-207,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：国家自然科学基金 (No :39870 2 84);全军医药卫生科研基金资助项目 (No :98M0 4 4 )资助

摘　　要：目的 :探讨创伤后应激障碍 (PTSD)精神与行为异常的病理生理基础。方法 :利用频率 2 5Hz、波宽 1ms、串长 10s、串隔 7min、强度 10 0 μA的恒流、单脉冲电流 ,反复惊厥阈下电刺激海马 ,并定量检测了实验动物海马组织匀浆及线粒体Na+ 、 K+ 、 ATP酶、Ca2 + 、 ATP酶活性改变。结果 :电刺激停止后 48h内实验动物海马细胞线粒体Na+ 、 K+ 、 ATP酶活性明显下降 ,P <0 0 5 ;72h内Ca2 + 、 ATP酶活性显著降低 ,P <0 0 5。结论 :海马组织 ,特别是海马细胞线粒体钠钾泵与钙泵功能受损 ,在实验动物长时程PTSD样情感行为异常发生发展中可能有重要意义。Objective: To explore the pathophysiological role in the pathogenesis of the lasting emotional behavior disorder following posttraumatic stress disorder(PTSD). Method: 72 male Wistar rats were divided randomly into three groups. Group SE (n=32) was for PTSD animal model by constant pulsating current of 100 μ A and intra-train frequencies of 25 Hz, pulsating duration of 1 ms, train duration of 10 s and stimulative interval of 7 min for 5 days with 8 times each day. Group CE(n=32) for control of electrode implanted in hippocampus without stimulation, and group NC(n=8) for normal control. The activities of Na+-K+-ATPase and Ca 2+-ATPase in hippocami of the animals were detected through neurochemical method. Result: It was showed that Na+-K+-ATPase activity in mitochondria of hippocampus cells in group SE decreased significantly (p<0.05) at 48 h after the last stimulation, and Ca 2+-ATPase did also at 72 h. Conclusion: It is indicated that the dysfunction of Na+-K+ pump and Ca 2+-ATPase in hippocampus, especially in its mitochondria, plays an important role in the development of PTSD.

关 键 词：应激障碍 创伤后 NA^+-K^+-ATP酶 CA^2+-ATP酶 海马 

分 类 号：R641[医药卫生—外科学]