重组人表皮生长因子促人角膜缘上皮细胞生长的研究  

Effect of Recombined Human Epidermal Growth Factor on Proliferation of Human Epithelial Cells

作　　者：施宇华[1] 曹凯[1] 刘虎[1] 陈永汉[1] 覃又文[2] 

机构地区：[1]南京医科大学第一附属医院眼科,江苏南京210029 [2]南京医科大学第一附属医院内分泌科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2002年第4期307-309,共3页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨重组表皮生长因子(rhEGF)对人角膜缘上皮细胞生长的影响。方法:采用rhEGF作用于体外培养的人角膜缘上皮细胞,记录细胞生长曲线,并用四唑氮盐(MTT)法测定细胞增殖状况。结果:rhEGF浓度为10ng/ml时对人角膜缘上皮细胞的促增殖作用最强(P<0.05)。MTT法48h的吸光度值实验组均高于对照组(P<0.01)。结论:rhEGF对体外培养人角膜上皮细胞有明显促增生作用。Objective:To explore the effect of recombined human epidermal growth factor (rhEGF) on proliferation of human corneal epithelial cells. Method:The rhEGF was added into cultured medium of human corneal epithelial cells. The sum of cells in different periods of corneal epithelial cells was recorded and the method of 3 [4,5 dimethylthiazol 2 yl] 2,5 diphenyltetrazolium bromide (MTT) assay was applied to estimate the state of proliferation. Results:The proliferation of human corneal epithelial cells can be promoted to the greatest extent by rhEGF at the concentration of 10 ng/ml (P< 0.05). In the experimental groups, the values of A of MTT assay at 48 h are higher than those in the control groups(P< 0.01). Conclusion:The rhEGF can promote the proliferation of human corneal epithelium.

关 键 词：表皮生长因子 人角膜上皮细胞 细胞培养 细胞增生 

分 类 号：R772.2[医药卫生—眼科]