人胚胎发育中期结肠神经系统的研究  

A study of human enteric nervous system development in the middle stage of embryogenesis

作　　者：宓开鸿[1] 李继承[1] 

机构地区：[1]浙江大学细胞生物学与组织工程学研究所,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2002年第3期181-184,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省分析测试基金 (990 75 ) ;浙江省教委科研基金 ;浙江省教委回国人员基金;浙江省优秀青年科技人才专项科研基金

摘　　要：目的 :观察人胚胎发育中期 (4个月～ 6个月 )结肠神经系统的发育。方法 :人胚胎结肠切片和铺片 ,用抗PGP9.5和抗 S- 10 0蛋白的 PAP免疫组织化学以及 NADPHd酶组织化学。结果 :1中期结肠肌间神经明显增多 ,PGP9.5阳性反应神经弥散分布 ,S- 10 0蛋白反应阳性神经呈竹篓样分布。粘膜下层中阳性神经逐渐分化 ,形成粘膜下深丛和浅丛。2氮能神经弥散分布于整个结肠壁肌间 ,环行肌层中阳性神经纤维与平滑肌纤维相平行。在粘膜下深层中偶见氮能核周体。 3铺片可见 ,反应阳性神经纤维形成复杂的肌间神经网络。结论 :中期是结肠神经系统发育的重要时期。Objective: To investigate the development of the enteric nervous system (ENS) in the middle stage (the 4th～6th months) of human embryogenesis.Methods: A histologic evaluation of the colonic enteric nervous system was done using NADPHd histochemistry,PAP immunohistochemistry,with anti PGP 9.5 and anti S 100 protein.Results: During this stage of embryology three things were noted.① The nerves in the myenteric layer increased markedly. This was shown by the PGP 9.5 immunoreactive nerves spreading out and the S 100 protein immunoreactive nerves forming a 'bamboo basket' pattern. ② The whole myenteric colon showed nitrigeric nerves paralleling the growth of the myofibers in the circular muscle layer. Nitrigeric perikara were rarely found in the extrinsic submucosal layer. ③ In the whole mounted preparations reactive nerves formed the complex nerve net in the myenteric layer. Conclusion: The middle stage of embryogensis is very important to the development of the colon ENS.

关 键 词：胚胎发育 结肠神经系统 胚胎学 ENS 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]