人乳腺癌细胞株透明质酸酶的表达及其意义  

Expression of hyaluronidase in human breast cancer cell lines and implication

作　　者：任国胜[1] 王小毅[1] 姚榛祥[1] 

机构地区：[1]重庆医科大学附属第一医院普外科,400016

出　　处：《中华实验外科杂志》2002年第4期336-337,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (30 0 70 2 92 )

摘　　要：目的　通过检测人乳腺癌细胞株透明质酸酶 (Hyse)水平的表达 ,并与雌激素受体(ER)、以及血管内皮生长因子 (VEGF)、基质金属蛋白酶 9(MMP 9)和组织蛋白酶 D(Cath D)比较 ,探讨Hyse在乳腺癌侵袭、转移及预后评价中的作用。 方法　应用细胞培养技术、酶联免疫吸附测定和免疫组织化学等方法分别测定 10株人乳腺癌细胞的Hyse ,以及VEGF、MMP 9、和Cath D的表达。结果　 10株人乳腺癌细胞Hyse的表达存在明显差异。ER(+ )细胞株Hyse表达低水平 ,ER(-)细胞株Hyse表达高水平 (P <0 .0 1)。Hyse高表达细胞株中VEGF、MMP 9和Cath D的表达明显高于Hyse低表达细胞株 (P <0 .0 5 )。结论　Hyse与乳腺癌的侵袭、转移潜能及预后有一定的相关性。Objective To evaluate the significance of hyaluronidase in infiltration,metastasis and prognosis of breast cancer through detecting the expression of hyaluronidase,vascular endothelial growth factor,matrix metalloproteninase 9,cathepsin D in human breast cancer cell lines and comparing it with estrogen receptor (ER).Methods Cell culture technique,enzyme linked sorbent assay (ELSA) and immunohistochemical staining were employed to detect the expression of hyaluronidase,vascular endothelial growth factor,matrix metalloproteinase 9 and cathepsine D in 10 human breast cancer cell lines,respectively.Results The expression levels of hyaluronidase in 10 cell lines varied significantly.with low expression in ER positive cell lines and high in ER negative ones(P<0.01).The expression of vascular endothelial growth factor,matrix metalloproteinase 9 and cathepsine D was significantly stronger in the cell lines showing higher Hyaluronidase level than those expressing lower hyaluronidase level (P< 0.05 ).Conclusion Hyaluronidase may play a role in infiltration,metatasis and prognosis of breast cancer.

关 键 词：人乳腺癌细胞株 透明质酸酶 雌激素受体 血管内皮生长因子 基质金属蛋白酶-9 组织蛋白酶-D 

分 类 号：R737.9[医药卫生—肿瘤]