大鼠实验性脑出血后脑组织中c-fos基因的表达与局部脑血流的改变  

Expression of c-fos gene in brain tissue and change of focal cerebral blood flow after experimental intracerebral hemorrhage in rats

作　　者：何效兵[1] 何家声[2] 姜亚军[2] 吴颢昕[3] 

机构地区：[1]连云港市第一人民医院神经内科,222002 [2]东南大学医学院附属中大医院神经内科 [3]南京中医药大学

出　　处：《临床神经病学杂志》2002年第3期132-135,共4页Journal of Clinical Neurology

摘　　要：目的　探讨大鼠实验性脑出血后脑组织中即刻早期基因c fos的表达和局部脑血流的变化。方法　采用Nath改良法建立大鼠脑出血模型 ;免疫组化法及RT PCR法测定其脑组织中fos蛋白和c fosmRNA的表达 ;氢清除法测定其局部脑血流。结果　大鼠血肿周围区 (基底节 )在脑出血后 1小时即出现fos蛋白的表达 ,至 3小时达高峰 ;c fosmRNA于出血后 1小时达表达高峰 ,至 3小时后仍有较高水平的表达 ;出血后 1小时全脑的血流量均下降 ,4小时恢复至对照组水平 ,并维持至出血后 2 4小时 ,随后的 2 4小时内再次出现脑血流下降。结论　大鼠脑出血后 ,血肿周围区和双侧皮质区的脑组织中存在着c fos基因的快速而长久的诱导表达。局部脑血流的下降相对短暂 ,且脑血流的下降在时程上与cObjective To explore the expression of c-fos gene at early stage and changes of focal cerebral blood flow (CBF) in brain tissue after intracerebral hemorrhage(ICH) in rats.Methods The rat models with ICH were made by Nath improvement method;fos protein in brain tissue and the expression of c-fos mRNA were measured by immunohistochemistry and RT-PCR method;its focal cerebral blood flow (CBF) was measured by hydrogen clearing method.Results The expression of fos protein was found at 1 h after ICH in the ipsilateral basal ganglia after ICH,it peaked at 3 h;c-fos mRNA peaked at 1 h after ICH,and had still expression of higher level after 3 h;cBF was reduced at 1 h after ICH,and returned to the level of the control group by 3 h and maintained to 24 h after ICH,and then appeared the reduction of CBF during 24 h again.Conclusion The increase and long induction expression of c-fos gene were showed in brain tissue of hematoma periperal zone and contralateral cortex one.Although focal CBF reduced after ICH in rats,it wasn't consistent with the expression of c-fos gene.

关 键 词：脑出血 FOS基因 脑血流 病理 

分 类 号：R743[医药卫生—神经病学与精神病学]