缺血再灌流时胶质源性神经营养因子在大鼠脑组织的分布特点  

The distributive characteristics of glial cell line-derived neurotrophic factor in brain tissue during ischemia-reperfusion in rats

作　　者：袁琼兰[1] 蓝顺清[1] 李瑞祥[2] 张光鹏[2] 羊惠君[2] 

机构地区：[1]泸州医学院解剖学教研室与神经生物学研究室,646000 [2]华西医科大学解剖学教研室

出　　处：《临床神经病学杂志》2002年第3期136-138,共3页Journal of Clinical Neurology

摘　　要：目的　观察大鼠脑缺血再灌流时胶质源性神经营养因子 (GDNF)在脑组织的分布特点 ,及其在缺血性脑损伤中的作用。方法　阻断大鼠大脑中动脉 (MCA)血流 2小时 ,再灌流 0 .5～ 48小时制成局灶性脑缺血模型 ,HE染色评价缺血性脑损伤的组织学特点 ,免疫组化法观察GDNF在脑组织的分布特点。结果　再灌流 0 .5小时组有灶性缺血区 ,2 4小时组面积最大 ,包括视前区、纹状体和皮质。再灌流 6小时组开始出现神经元不可逆变性 ,2 4小时组梗死形成。再灌流 0 .5小时组 ,缺血区皮质神经元GDNF弱阳性 ,缺血周边区中等阳性 ;再灌流 3～ 48小时组 ,缺血区神经元GDNF阴性。再灌流 48小时组视前区的梗死周边区巨噬细胞GDNF呈强阳性。GDNF阳性细胞计数显示缺血区各实验组与正常组相比均减少 (均P <0 .0 1) ;2 4小时和 48小时组分别与 0 5小时组和 3小时组相比 ,GDNF阳性细胞数减少 (分别P <0 .0 1)。结论　缺血性脑损伤时 ,变性死亡的神经元GDNF不表达 。Objective To observe the distributive characteristics of glial cell line-derived neurotrophic factor(GDNF)in brain tissue during cerebral ischemia-reperfusion in rats,and its role in ischemic brain damage.Methods The model of focal cerebral ischemia was made by occluding middle cerebral artery(MCA) for 2 h and reperfusion for 0.5～48 h, HE Staining was used to investigate the histological features of ischemic cerebral damage,the immunohistochemical method was used to observe the distributive characteristics of GDNF in brain tissue in rats. Results The focal ischemic areas (including preoptic area, striatum and cortex) presented at 0.5 h of reperfusion and peaked at 24 h. The neurons presented irreversible degeneration at 6 h of reperfusion. At 24 h, the ischemic area in the preoptic area developed into infarct form. At 0.5 h of reperfusion, neurons in ischemic cortex showed GDNF weak positive, neurons in peri-ischemic regions showed GDNF moderate positive. During reperfusion 3～48 h, neurons in ischemic regions showed GDNF negative. Up to 48 h of reperfusion, the active microglias or macroglias in periinfart area strongly expressed GDNF.The number of GDNF positive cells in all groups decreased (P< 0.01);the number of GDNF positive cells in comparison of 24 h and 48 h with the 0.5 h and 3 h of reperfusion decreased (P< 0.01).Conclusion During ischemic cerebral damage, the degenerative and died neruons doesn't express GDNF protein.The survival neurons and early active microglias or macroglias would have a stronger expression of GDNF.

关 键 词：胶质源性神经营养因子 脑缺血再灌注 神经元 小胶质细胞 

分 类 号：R741.02[医药卫生—神经病学与精神病学]