宫颈癌与多种病原微生物感染、细胞因子及硒元素含量相关性研究  

The relationship of cervical cancer with pathogen infectious,cytokine and Se

作　　者：郑曙民[1] 张春玲[1] 李连青[2] 朱庆义[2] 韩存芝[3] 荆洁线[3] 

机构地区：[1]山西省肿瘤医院,太原030013 [2]山西省儿童医院 [3]山西省肿瘤研究所

出　　处：《中华实验和临床病毒学杂志》2002年第2期179-183,共5页Chinese Journal of Experimental and Clinical Virology

基　　金：山西省自然科学资金(9710 6 2 )

摘　　要：目的　探讨多种病毒及支原体性病原微生物感染、细胞因子和微量元素硒 (Se)含量与宫颈癌发病的相关性。方法　PCR法检测宫颈癌组织中人乳头瘤病毒、疱疹病毒、人巨细胞病毒、EB病毒、沙眼衣原体和解脲支原体的感染 (75份非宫颈癌患者正常宫颈组织作对照 ) ;ELISA法检测血清中细胞因子IL 2R、TNF的含量 ;荧光光度技术检测血清中硒 (Se)元素含量。结果　在 1 95份宫颈癌患者的癌组织标本中 ,病原微生物感染阳性者为 1 66份 (85 1 % ) ,其中 1 6 ,1 8和 35型人乳头瘤病毒感染60份 (30 8% ) ;6和 1 1型人乳头瘤病毒感染 5份 (2 6 % ) ;2型单纯疱疹病毒感染 60份 (30 8% ) ;1型单纯疱疹病毒感染 4份 (2 1 % ) ;EB病毒感染 3份 (1 5 % ) ;人巨细胞病毒感染 6份 (3 1 % ) ;沙眼衣原体感染 1 1份 (5 6 % )和解脲支原体感染 1 7份 (8 7% )。对照组 75份正常宫颈组织中病原微生物感染阳性者为 1 5份 (2 0 0 % ) ,其中 ,1 6 ,1 8和 35型人乳头瘤病毒感染 3份 (4 0 % ) ;2型单纯疱疹病毒感染5份 (6 7% ) ;EB病毒感染 1份 (1 3 % ) ;沙眼衣原体感染 3份 (4 0 % )和解脲支原体感染 3份 (4 0 % )。62份宫颈癌和 36份正常人血清中 ,白细胞介素 2受体 ( x =356 44U ml)和肿瘤坏死因子 ( x =373 48pg ml)浓度均比?Objective To identify the relationship of cervical cancer with pathogen infectious,cytokine and Se.Methods On the one hand regarded tissues of the carcinoma of the uterine cervix with 195 caues as the experimental group and ordinary cervical tissues with 75 caues as the control group.Polymerase chain reaction (PCR) to derect them.On the other hand applied ELISA to detect cytokine IL 2R,TNF and fluorescent laminosity technique to detect element Se in the serums.Results In the experimental group those infected pathogen were 166 caues (85.1%),In all of pathogen HPV 16,18,35 type were 60 coues (30 8%),HSV 2 were 60 cases (30 8%),While those ordinary tissues infected pathogen were 15 caues (20 0%),in the contrast group.HPV 16,18,35 type and HSV 2 mere 4.0% and 6.7% respectively,( P <0 001).In the serums of effective 62 experimental objects IL 2R ( =356.44 U/ml) and TNF ( =373.48 pg/ml) were much high than them in the serums of effective 36 contrast objects ( P <0 001).But Se ( =0 058 mg/ml) was lower than it in the serims of contrast objects ( P <0 05).Conclusion The occurrence of carcinoma of the uterine cercix is colsely connected with infection of HPV 16,18,35 and HSV 2,high level of cellular factirs IL 2R,TNF and low level of element Se.

关 键 词：宫颈肿瘤 人乳头状瘤病毒 单纯疱疹 硒 细胞活素类 

分 类 号：R737.33[医药卫生—肿瘤]